Nitric Oxide and Hydrogen Peroxide Production are Involved in Systemic Drought Tolerance Induced by 2R,3R-Butanediol in Arabidopsis thaliana.
Nitric Oxide and Hydrogen Peroxide Production are Involved in Systemic Drought Tolerance Induced by 2R,3R-Butanediol in Arabidopsis thaliana.
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DOI：
10.5423/ppj.oa.07.2013.0069
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发表时间：
2013-12
期刊：
影响因子：
--
通讯作者：
Kim YC
中科院分区：
文献类型：
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作者：
Cho SM;Kim YH;Anderson AJ;Kim YC
2 R，3R-丁醇是由某些根际细菌产生的挥发性化合物，通过气孔关闭机制参与拟南芥的诱导耐旱性。在这项研究中，我们研究了一氧化氮和过氧化氢在诱导耐旱性的参与，因为这些都是由脱落酸（阿坝）介导的干旱胁迫反应中的信号传导剂。基于代谢的分析表明，系统性一氧化氮和过氧化氢的产生是由2 R，3R-丁二醇诱导的，并且与编码硝酸还原酶和一氧化氮合酶的基因的表达相关。共处理的2 R，3R-丁二醇与硝酸还原酶抑制剂或一氧化氮合酶抑制剂降低一氧化氮的产生，减少诱导耐旱性。通过2 R，3R-丁二醇与NADPH氧化酶或过氧化物酶抑制剂的共处理来消除过氧化氢的增加。这些研究结果支持由某些根际细菌合成的挥发性2 R，3R-丁二醇通过涉及一氧化氮和过氧化氢产生的机制在诱导耐旱性中是积极的参与者。
2R,3R-Butanediol, a volatile compound produced by certain rhizobacteria, is involved in induced drought tolerance in Arabidopsis thaliana through mechanisms involving stomatal closure. In this study, we examined the involvement of nitric oxide and hydrogen peroxide in induced drought tolerance, because these are signaling agents in drought stress responses mediated by abscisic acid (ABA). Fluorescence-based assays showed that systemic nitric oxide and hydrogen peroxide production was induced by 2R,3R-butanediol and correlated with expression of genes encoding nitrate reductase and nitric oxide synthase. Co-treatment of 2R,3R-butanediol with an inhibitor of nitrate reductase or an inhibitor of nitric oxide synthase lowered nitric oxide production and lessened induced drought tolerance. Increases in hydrogen peroxide were negated by co-treatment of 2R,3R-butanediol with inhibitors of NADPH oxidase, or peroxidase. These findings support the volatile 2R,3R-butanediol synthesized by certain rhizobacteria is an active player in induction of drought tolerance through mechanisms involving nitric oxide and hydrogen peroxide production.