Age and exercise training alter signaling through reactive oxygen species in the endothelium of skeletal muscle arterioles.
Age and exercise training alter signaling through reactive oxygen species in the endothelium of skeletal muscle arterioles.
复制标题
年龄和运动训练会改变骨骼肌小动脉内皮细胞中活性氧的信号传导。
DOI：
10.1152/japplphysiol.00341.2012
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Muller-Delp,JudyM
中科院分区：
文献类型：
--
作者：
Sindler,AmyL;Reyes,Rafael;Chen,Bei;Ghosh,Payal;Gurovich,AlvaroN;Kang,LoriS;Cardounel,ArturoJ;Delp,MichaelD;Muller-Delp,JudyM
Exercise training ameliorates age-related impairments in endothelium-dependent vasodilation in skeletal muscle arterioles. Additionally, exercise training is associated with increased superoxide production. The purpose of this study was to determine the role of superoxide and superoxide-derived reactive oxygen species (ROS) signaling in mediating endothelium-dependent vasodilation of soleus muscle resistance arterioles from young and old, sedentary and exercise-trained rats. Young (3 mo) and old (22 mo) male rats were either exercise trained or remained sedentary for 10 wk. To determine the impact of ROS signaling on endothelium-dependent vasodilation, responses to acetylcholine were studied under control conditions and during the scavenging of superoxide and/or hydrogen peroxide. To determine the impact of NADPH oxidase-derived ROS, endothelium-dependent vasodilation was determined following NADPH oxidase inhibition. Reactivity to superoxide and hydrogen peroxide was also determined. Tempol, a scavenger of superoxide, and inhibitors of NADPH oxidase reduced endothelium-dependent vasodilation in all groups. Similarly, treatment with catalase and simultaneous treatment with tempol and catalase reduced endothelium-dependent vasodilation in all groups. Decomposition of peroxynitrite also reduced endothelium-dependent vasodilation. Aging had no effect on arteriolar protein content of SOD-1, catalase, or glutathione peroxidase-1; however, exercise training increased protein content of SOD-1 in young and old rats, catalase in young rats, and glutathione peroxidase-1 in old rats. These data indicate that ROS signaling is necessary for endothelium-dependent vasodilation in soleus muscle arterioles, and that exercise training-induced enhancement of endothelial function occurs, in part, through an increase in ROS signaling.
