Molecular evaluation of diamide resistance in diamondback moth (Lepidoptera: Yponomeutidae) populations using quantitative sequencing
Molecular evaluation of diamide resistance in diamondback moth (Lepidoptera: Yponomeutidae) populations using quantitative sequencing
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DOI：
10.1007/s13355-017-0482-3
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发表时间：
2017-05-01
影响因子：
1.3
通讯作者：
Jouraku, A.
中科院分区：
文献类型：
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作者：
Sonoda, S.;Inukai, K.;Jouraku, A.
摘要兰尼定受体基因G4946E突变是小菜蛾对联胺类杀虫剂产生抗药性的主要机制之一，建立了一种用定量测序方法估计小菜蛾群体兰尼定受体基因G4946E突变的方法。使用基于QS的方法，使用现场收集的人群来检验G4946E突变比例与联胺(氟苯二胺)死亡率之间的关系。结果表明，这些种群可归类为联胺抗性种群。
A method to estimate proportions of the G4946E mutation in the ryanodine receptor gene, a major mechanism of diamide insecticide resistance, was developed for Plutella xylostella Linnaeus populations using quantitative sequencing (QS). Using the QS-based method, the relationship between proportions of the G4946E mutation and mortalities for diamide (flubendiamide) was examined using field-collected populations. Results showed that the populations might be classifiable as diamide resistant.