Optogenetic control of freely behaving adult Drosophila using a red-shifted channelrhodopsin
Optogenetic control of freely behaving adult Drosophila using a red-shifted channelrhodopsin
复制标题
DOI：
--
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Hidehiko K. Inagaki;Yonil Jung;Eric D. Hoopfer;A. Wong;Neeli Mishra;John Y. Lin;R. Tsien;D. Anderson
中科院分区：
文献类型：
--
作者：
Hidehiko K. Inagaki;Yonil Jung;Eric D. Hoopfer;A. Wong;Neeli Mishra;John Y. Lin;R. Tsien;D. Anderson
光遗传学允许以毫秒级的精度操纵自由运动动物的神经活动，但其在果蝇中的应用受到限制。在这里，我们表明，最近描述的红色可激活的视紫红质（ReaChR）允许控制复杂的行为在自由移动的成年苍蝇，在波长不被认为是干扰正常的视觉功能。该工具提供了在刺激强度的宽动态范围内控制神经活动的机会。使用时间分辨激活，我们表明，男性求爱歌曲的神经控制可以分为概率，持久性和确定性，命令般的组件。前者（而非后者）的神经元受到社会经验的功能调节，这支持了它们构成了状态依赖影响的轨迹的观点。这种分离是不明显的，使用热发生工具，强调了时间上精确控制神经元激活的果蝇神经回路的功能解剖的重要性。
Optogenetics allows the manipulation of neural activity in freely moving animals with millisecond precision, but its application in Drosophila has been limited. Here we show that a recently described Red activatable Channelrhodopsin (ReaChR) permits control of complex behavior in freely moving adult flies, at wavelengths that are not thought to interfere with normal visual function. This tool affords the opportunity to control neural activity over a broad dynamic range of stimulation intensities. Using time-resolved activation, we show that the neural control of male courtship song can be separated into probabilistic, persistent and deterministic, command-like components. The former, but not the latter, neurons are subject to functional modulation by social experience, supporting the idea that they constitute a locus of state-dependent influence. This separation is not evident using thermogenetic tools, underscoring the importance of temporally precise control of neuronal activation in the functional dissection of neural circuits in Drosophila .