The Effects of Oral Rehmannia glutinosa Polysaccharide Administration on Immune Responses, Antioxidant Activity and Resistance Against Aeromonas hydrophila in the Common Carp, Cyprinus carpio L
The Effects of Oral Rehmannia glutinosa Polysaccharide Administration on Immune Responses, Antioxidant Activity and Resistance Against Aeromonas hydrophila in the Common Carp, Cyprinus carpio L
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口服地黄多糖对鲤鱼免疫反应、抗氧化活性和抗嗜水气单胞菌的影响
DOI：
10.3389/fimmu.2020.00904
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发表时间：
2020-05-08
影响因子：
7.3
通讯作者：
Nie, Guo-xing
中科院分区：
文献类型：
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作者：
Feng, Jun-chang;Cai, Zhong-liang;Nie, Guo-xing
研究了经口给予红藻多糖（RGP-1）对鲤鱼免疫调节特性、抗氧化活性和抗嗜水气单胞菌能力的影响。考察将纯化的RGP-1（250、500和1，000 μg/mL）与鲤鱼头肾细胞共培养。测定头肾细胞的增殖和吞噬活性，以及培养液中一氧化氮（NO）和细胞因子的浓度。接下来，将300条鲤鱼（47.66 ± 0.43 g）随机分为五组;两个对照组（阴性和阳性）施用无菌PBS，三个治疗组施用不同浓度的RGP-1（250、500和1，000 μg/mL），持续7天。随后，阳性组和治疗组感染A。阴性组给予无菌PBS 24 h。测定了鲤鱼血清中NO、细胞因子、溶菌酶（LZM）、碱性磷酸酶（AKP）的含量，肝胰脏中总抗氧化能力（T-AOC）、丙二醛（MDA）、谷胱甘肽（GSH）的含量以及超氧化物歧化酶（T-SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）的总活性。体外实验发现，RGP-1能显著增强细胞增殖和吞噬功能（P < 0.05），并诱导NO、促炎细胞因子（TNF-α、IL-1β、IL-6、IL-12）和抗炎细胞因子（IL-10、TGF-β）的产生（P < 0.05）。体内实验结果显示，与A.支原体感染（P < 0.05）。感染后NO、促炎细胞因子、LZM和AKP活性均明显低于阳性组（P < 0.05）。但无论感染与否，RGP-1治疗组的抗炎细胞因子（IL-10、TGF-β）表达均显著增加（P < 0.05）。提示RGP-1具有增强非特异性免疫功能、抗氧化活性和抗A.可作为一种安全有效的饲料添加剂应用于水产养殖。
The effects of the oral administration of Rehmannia glutinosa polysaccharide (RGP-1) on the immunoregulatory properties, antioxidant activity, and resistance against Aeromonas hydrophila in Cyprinus carpio L. were investigated. The purified RGP-1 (250, 500, and 1,000 μg/mL) was co-cultured with the head kidney cells of the common carp. The proliferation and phagocytosis activities of the head kidney cells, and the concentration of nitric oxide (NO) and cytokines in the culture medium were determined. Next, 300 common carps (47.66 ± 0.43 g) were randomly divided into five groups; the two control groups (negative and positive) were administered sterile PBS and the three treatment groups were administered different concentrations of RGP-1 (250, 500, and 1,000 μg/mL) for seven days. Subsequently, the positive and treatment groups were infected with A. hydrophila, and the negative group was administered sterile PBS for 24 h. The concentration of NO, cytokines, lysozyme (LZM), and alkaline phosphatase (AKP) in serum, the total antioxidant capacity (T-AOC), the levels of malonaldehyde (MDA) and glutathione (GSH), and the total activities of superoxide dismutase (T-SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in the hepatopancreas of the common carp were tested. We observed that RGP-1 could significantly enhance the proliferation and phagocytosis activities (P < 0.05), besides inducing the production of NO, pro-inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-12) and anti-inflammatory cytokines (IL-10, TGF-β) (P < 0.05) in vitro. The in vivo experimental results revealed that RGP-1 significantly enhanced NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-12), LZM and AKP activities, and the antioxidant content (T-AOC, SOD, CAT, GSH, GSH-Px, and MDA) compared to that observed in the negative group prior to A. hydrophila infection (P < 0.05). NO, pro-inflammatory cytokines, LZM and AKP activities were significantly lower than that in the positive group after infection (P < 0.05). However, whether infected or not, the expression of anti-inflammatory cytokines (IL-10, TGF-β) increased significantly in the RGP-1-treated groups (P < 0.05). Therefore, the results suggested that RGP-1 could enhance the non-specific immunity, antioxidant activity and anti-A. hydrophila activity of the common carp, and could be used as a safe and effective feed additive in aquaculture.