Temporal and spatial expression of flavonoid biosynthetic genes in flowers of Anthurium andraeanum
Temporal and spatial expression of flavonoid biosynthetic genes in flowers of Anthurium andraeanum
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DOI：
10.1111/j.1399-3054.2004.00402.x
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发表时间：
2004-11-01
影响因子：
6.4
通讯作者：
Davies, KM
中科院分区：
文献类型：
--
作者：
Collette, VE;Jameson, PE;Davies, KM
以红掌（Anthurium andraeanum）为材料，研究花色形成过程中花色素苷合成相关基因的表达.从火鹤佛焰苞的mRNA中构建cDNA文库，并鉴定了类黄酮生物合成酶查尔酮合成酶（CHS）、黄烷酮3-羟化酶（F3 H）、二氢黄酮醇4-还原酶（DFR）和花青素合成酶（ANS）的全长cDNA克隆。这些被用来测量花发育期间佛焰苞、不同花色品种、昼夜周期和佛焰苞中的转录水平。CHS、F3 H和ANS在佛焰苞和肉穗花序发育的各个时期均有表达。然而，DFR转录水平在不同阶段之间变化显著，DFR可能代表调控的关键点。在佛焰苞的DFR转录丰度的昼夜节律也被观察到，与转录水平高，在黎明和黄昏和中午低。花青苷生物合成的控制红掌佛焰苞不同于其他物种的花，与DFR的一个关键的调节点和一个复杂的混合的发展和环境控制信号。
The expression of anthocyanin biosynthesis genes during flower colour development in Anthurium andraeanum (anthurium) was studied. A cDNA library was constructed from mRNA from the anthurium spathe, and full-length cDNA clones identified for the flavonoid biosynthetic enzymes chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), dihydroflavonol 4-reductase (DFR) and anthocyanidin synthase (ANS). These were used to measure transcript levels in the spathe during flower development, in cultivars with different flower colours, over the diurnal cycle, and in the spadix. CHS, F3H and ANS were expressed at all stages of spathe and spadix development. However, DFR transcript levels varied significantly between stages, and DFR may represent a key point of regulation. A diurnal rhythm of DFR transcript abundance in the spathe was also observed, with transcript levels high at dawn and dusk and low at noon. Control of anthocyanin biosynthesis in anthurium spathe differs from that described for flowers of other species, with DFR a key regulatory point and a complex mix of developmental and environmental control signals.