High performance preparative column chromatography of lipids using a new porous silica, iatrobeads® I. Separation of molecular species of sphingoglycolipids
High performance preparative column chromatography of lipids using a new porous silica, iatrobeads® I. Separation of molecular species of sphingoglycolipids
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使用新型多孔二氧化硅 itrobeads® I 进行脂质的高性能制备柱色谱。 鞘糖脂分子种类的分离
DOI：
10.1016/0005-2760(76)90054-0
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发表时间：
1976
期刊：
影响因子：
--
通讯作者：
Nagai Yoshitaka
中科院分区：
文献类型：
--
作者：
Ando Susumu;Isobe Miyoko;Nagai Yoshitaka
用全多孔硅胶球（Iatrobeads）柱层析法对人红细胞中性糖脂进行了良好的制备性分离。在大气压下，通过Iatrobeads色谱柱的溶剂流速较快，因此洗脱带在色谱柱中迁移，扩散最小。神经酰胺二己糖苷和神经酰胺三己糖苷在Iatrobeads柱上均分离成两种不同分子种类的级分。两个神经酰胺四糖，globoside I和paragloboside，也清楚地分离的Iatrobeads柱。化学分析表明，这些分子种类的分离是由于它们的脂肪酸和长链碱组成的差异。发现鞘氨醇和植物鞘氨醇是人红细胞糖脂中长链碱基的次要成分。
Good preparative separation of neutral glycolipids of human erythrocytes was achieved by column chromatography using the totally porous silica spheres, Iatrobeads. The solvent flow through the Iatrobeads column was rapid under atmospheric pressure, and so elution bands migrated with minimal diffusion in the column. Ceramide dihexoside and ceramide trihexoside were both separated into two fractions of different molecular species on the Iatrobeads column. Two ceramide tetrasaccharides, globoside I and paragloboside, were also clearly separated on the Iatrobeads column. Chemical analysis showed that separation of these molecular species was due to differences in their fatty acid and long chain base compositions. Sphingadienine and phytosphingosine were found as minor components of long chain bases in human erythrocyte glycolipids.