CONNEXIN-43 AND CONNEXIN-40 GAP JUNCTIONAL PROTEINS ARE PRESENT IN ARTERIOLAR SMOOTH-MUSCLE AND ENDOTHELIUM IN-VIVO
CONNEXIN-43 AND CONNEXIN-40 GAP JUNCTIONAL PROTEINS ARE PRESENT IN ARTERIOLAR SMOOTH-MUSCLE AND ENDOTHELIUM IN-VIVO
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DOI：
10.1152/ajpheart.1995.268.2.h729
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发表时间：
1995-02-01
影响因子：
4.8
通讯作者：
DULING, BR
中科院分区：
文献类型：
--
作者：
LITTLE, TL;BEYER, EC;DULING, BR
应用间接免疫荧光技术结合激光共聚焦显微镜观察血管内皮细胞和血管内皮细胞连接蛋白43(Cx43)和连接蛋白40(Cx40)的分布。Cx43和Cx40在血管内皮细胞和血管内皮细胞中表达。在大鼠脑和提睾肌的微血管样本以及仓鼠颊囊小动脉中也发现了类似的染色模式。双标记研究表明，Cx40和Cx43高度共定位，表明在单个连接斑块中存在多个连接蛋白。定量比较了大鼠脑小动脉内皮细胞和平滑肌的荧光模式。Cx43和Cx40斑块直径在内皮层分别为0.9+/-0.1和0.8+/-0.1(SE)微米，在平滑肌分别为0.5+/-0.1和0.5+/-0.1微米。Cx43和Cx40在血管内皮细胞和血管中的平均斑块直径无差异。然而，内皮细胞的斑块明显大于血管内皮细胞的斑块(P<；0.05)。这些发现证明了来自三个不同组织的小动脉壁的两种细胞类型的细胞间通讯的潜力。数据还表明，内皮细胞内的偶联程度更高。
The distributions of connexin 43 (Cx43) and connexin 40 (Cx40) in smooth muscle and endothelium of resistance vessels were examined using indirect immunofluorescence techniques coupled with confocal microscopy. Cx43 and Cx40 were found in smooth muscle and endothelium. Similar staining patterns were found in microvessel samples from brain and cremaster of the rat and from arterioles of the hamster cheek pouch. Double-labeling studies showed a high degree of colocalization of Cx40 with Cx43, suggesting the presence of multiple connexins within a single junctional plaque. Quantitative comparisons were made of the fluorescent patterns in the endothelium and smooth muscle of rat brain arterioles. Cx43 and Cx40 plaque diameters were 0.9 +/- 0.1 and 0.8 +/- 0.1 (SE) mu m, respectively, in the endothelial layer and 0.5 +/- 0.1 and 0.5 +/- 0.1 mu m, respectively, in the smooth muscle. There was no difference between mean plaque diameters of Cx43 and Cx40 in endothelium or smooth muscle. However, plaques were significantly larger in endothelium than in smooth muscle (P < 0.05). These findings demonstrate the potential for cell-cell communication in both cell types of the wall of arterioles from three different tissues. The data also suggest a greater level of coupling within the endothelium.