Parallel adaptive evolution cultures of Escherichia coli lead to convergent growth phenotypes with different gene expression states
Parallel adaptive evolution cultures of Escherichia coli lead to convergent growth phenotypes with different gene expression states
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DOI：
10.1101/gr.3832305
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发表时间：
2005-10-01
期刊：
影响因子：
7
通讯作者：
Palsson, BO
中科院分区：
文献类型：
--
作者：
Fong, SS;Joyce, AR;Palsson, BO
实验室进化可以用来解决适应选择压力的基本问题，并最终解决进化过程。在这项研究中，我们调查了适应性进化过程中的生长表型和全局基因表达状态的再现性。对大肠杆菌K-12 MG 1655在乳酸和甘油基本培养基上进行的平行重复适应性进化实验结果表明：（1）进化终点的生长表型是趋同的和可重复的;和（3）进化基因表达反应包括大量的补偿性表达变化和较少数量的适应性有益表达变化，这些变化在进化株中是常见的。基因表达的变化最初显示了大量的差异表达的基因，以响应环境的变化，然后大多数基因的基线表达水平的回报，留下一个相对较小的差异表达的基因组在进化种群之间的变化在终点。
Laboratory evolution can be used to address fundamental questions about adaptation to selection pressures and, ultimately, the process of evolution. In this Study, we investigated the reproducibility of growth phenotypes and global gene expression states during adaptive evolution. The results from parallel, replicate adaptive evolution experiments of Escherichia coli K-12 MG1655 grown on either lactate or glycerol minimal media showed that (1) growth phenotypes at the endpoint Of evolution are convergent and reproducible; (2) endpoints of evolution have different underlying gene expression states; and (3) the evolutionary gene expression response involves a large number of compensatory expression changes and a smaller number of adaptively beneficial expression changes common across evolution strains. Gene expression changes initially showed a large number of differentially expressed genes in response to an environmental change followed by a return of most genes to a baseline expression level, leaving a relatively small set of differentially expressed genes at the endpoint that varied between evolved populations.