Dielectrophoretic investigation of plant virus particles: Cow Pea Mosaic Virus and Tobacco Mosaic Virus
Dielectrophoretic investigation of plant virus particles: Cow Pea Mosaic Virus and Tobacco Mosaic Virus
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DOI：
10.1002/elps.200500928
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发表时间：
2006-10-01
期刊：
影响因子：
2.9
通讯作者：
Morgan, Hywel
中科院分区：
文献类型：
--
作者：
Ermolina, Irina;Milner, Joel;Morgan, Hywel
本文报道了两种不同几何形状的无包膜植物病毒，即豌豆花叶病毒(CPMV)和烟草花叶病毒(TMV)的介电泳法(DEP)行为的实验结果。还报道了相同尺寸的羧基改性乳胶珠的DEP性能。通过测量粒子上的DEP力变为零的频率(交叉频率)，得到了单个粒子的DEP特性。利用图像处理技术测量了粒子系综的DEP行为。根据DEP数据对粒子的介电性能进行了评估。结果表明，CPMV的表面电导为0.3 ns，TMV的表面电导为0.38 ns，直径为27 nm的羧基胶乳微珠的表面电导为0.52 ns。数据分析表明，分离烟草花叶病毒和烟草花叶病毒的最佳条件是低电导率悬浮介质--低于1ms/m。
This paper reports experimental results on the dielectrophoretic (DEP) behaviour on two nonenveloped plant viruses of different geometrical shapes, namely Cow Pea Mosaic Virus (CPMV) and Tobacco Mosaic Virus (TMV). The DEP properties of carboxy-modified latex beads of the same size are also reported. The DEP properties of single particles were obtained from measurement of the frequency at which the DEP force on a particle goes to zero (the crossover frequency). The DEP behaviour of particle ensembles was also measured using image processing. The dielectric properties of the particles were evaluated from the DEP data. The surface conductance was found to be 0.3 nS for CPMV, 0.38 nS for TMV, and 0.52 nS for 27 nm diameter carboxy-latex beads. Data analysis has shown that the optimal condition for separation of TMV and CPMV is a low-conductivity suspending medium - below 1 mS/m.