Differential induction and localization of mPer1 and mPer2 during advancing and delaying phase shifts.
Differential induction and localization of mPer1 and mPer2 during advancing and delaying phase shifts.
复制标题
mPer1 和 mPer2 在提前和延迟相移期间的差分感应和定位。
DOI：
10.1046/j.1460-9568.2002.02224.x
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Silver,Rae
中科院分区：
文献类型：
--
作者：
Yan,Lily;Silver,Rae
短暂的光暴露以相位依赖的方式重置哺乳动物生物钟的机制尚不清楚，但被认为涉及Pergene表达。在行为水平上，光脉冲在主观夜间早期产生相位延迟，在主观夜间晚期产生相位提前，并且在主观夜间中期或主观白天没有相移。为了了解Pergene活性与行为相移之间的关系，我们在主观夜间检查了小鼠视交叉上核（SCN）中光诱导的mPer1和mPer2表达，以了解SCN异质性。在SCN的VIP区域（称为“核心”），光诱导的mPer1表达发生在主观夜间的所有时间，而emPer2诱导仅见于主观夜间早期。在SCN的其余区域（称为“壳”），相位延迟光脉冲产生nomPer1但显著mPer2表达，而相位提前光脉冲产生nomPer2但实质mPer1诱导。此外，在主观夜间的光脉冲之后，在壳体中诱导出neithermPer1normPer2。结果表明，只有当光诱导的Pergene表达从核心扩散到壳SCN时，才会发生行为相移，壳中的mPer1表达对应于相位提前，mPer2对应于相位延迟。结果表明，SCN中光诱导的Pergene表达的时间过程和定位揭示了SCN内通信的重要方面。
The mechanism whereby brief light exposure resets the mammalian circadian clock in a phase dependent manner is not known, but is thought to involvePergene expression. At the behavioural level, a light pulse produces phase delays in early subjective night, phase advances in late subjective night, and no phase shifts in mid‐subjective night or subjective day. To understand the relationship betweenPergene activity and behavioural phase shifts, we examined light‐inducedmPer1andmPer2expression in the suprachiasmatic nucleus (SCN) of the mouse, in the subjective night, with a view to understanding SCN heterogeneity. In theVIP‐containing region of the SCN (termed ‘core’), light‐inducedmPer1expression occurs at all times of the subjective night, whilemPer2induction is seen only in early subjective night. In the remaining regions of the SCN (termed ‘shell’), a phase delaying light pulse produces nomPer1but significantmPer2expression, while a phase advancing light pulse produces nomPer2but substantialmPer1induction. Moreover, following a light pulse during mid‐subjective night, neithermPer1normPer2are induced in the shell. The results reveal that behavioural phase shifts occur only when light‐inducedPergene expression spreads from the core to the shell SCN, withmPer1expression in shell corresponding to phase advances, andmPer2corresponding to phase delays. The results indicate that the time course and the localization of light‐inducedPergene expression in SCN reveals important aspects of intra‐SCN communication.