Ontogeny of IgM-bearing cells and changes in the immunoglobulin M-like protein level (IgM) during larval stages in sea bass (Dicentrarchus labrax)
Ontogeny of IgM-bearing cells and changes in the immunoglobulin M-like protein level (IgM) during larval stages in sea bass (Dicentrarchus labrax)
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DOI：
10.1006/fsim.1996.0061
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发表时间：
1997-01-01
影响因子：
4.7
通讯作者：
Romestand, B
中科院分区：
文献类型：
--
作者：
Breuil, G;Vassiloglou, B;Romestand, B
免疫荧光染色(FITC)和流式细胞仪分析(FAGS)免疫荧光染色(FITC)和流式细胞仪(FAGS)免疫荧光染色(FITC)和流式细胞仪(FAGS)染色的细胞的个体发育的描述。此外，用灵敏的ELISA法测定了幼虫的免疫球蛋白(IgM)水平，并对淋巴器官的发育进行了组织学研究。用ELISA法检测到少量的IgM，在孵化后第5天呈规律性下降，第5天至第15天未检测到IgM，第15天至第30天再次检测到IgM，FAGS检测到IgM阳性细胞。在此期间，胸腺和头肾变成淋巴样。从30d到95日龄，IgM水平升高，并与仔鱼的体重呈正相关。这些结果表明，从第0天到第5天检测到的IgM来自母鱼，而从第15天检测到的IgM是新合成的IgM。此外，当淋巴器官完全发育时，50日龄的鳕鱼幼体中存在成熟的B细胞和较强的IgM合成。(C)1997年学术出版社有限公司。
The ontogenetic development of IgM positive cells was described as demonstrated by immune-fluorescent staining (FITC) and flow cytometric analysis (FAGS) of cells stained for IgM expression with a mouse anti-sea bass IgM monoclonal antibody. In addition, the immunoglobulin (IgM) level in larvae was measured using a sensitive ELISA and the development of the lymphoid organs was studied histologically. A small amount of IgM was detected by ELISA in the eggs, decreasing regularly until day 5 post-hatching, with no IgM being detected from day 5 to day 15. IgM was again detectable from day 15 to day 30 together with the detection of IgM positives cells by FAGS analysis. During this period the thymus and the head kidney became lymphoid. From day 30 to day 95, the IgM level increased and correlated with the body weight of the larvae. These results show that the IgM detected from day 0 to day 5 derive from maternal fish while the IgM detected from day 15 are neo-synthesised IgM. Moreover, mature B cells and strong IgM synthesis are present in 50-day-old sea bass larvae when the lymphoid organs are fully developed. (C) 1997 Academic Press Limited.