Characterization of a family of monoclonal antibodies which bind Schistosoma japonicum egg antigens and express an interstrain cross-reactive idiotype.
Characterization of a family of monoclonal antibodies which bind Schistosoma japonicum egg antigens and express an interstrain cross-reactive idiotype.
复制标题
结合日本血吸虫卵抗原并表达株间交叉反应独特型的单克隆抗体家族的表征。
DOI：
10.1111/j.1365-3024.1990.tb00948.x
复制
发表时间：
1990
影响因子：
2.2
通讯作者：
Kresina,TF
中科院分区：
文献类型：
--
作者：
Olds,GR;Kresina,TF
P3NS1骨髓瘤细胞与来自自然感染或超免小鼠的淋巴样细胞进行体细胞融合，获得一系列单抗(Moab)。用日本血吸虫自然感染C57BL/6小鼠，用制备的OFS免疫BALB/c小鼠。日本不溶性卵抗原(SEA)。从抗体亚型、抗原结合特异性、对抗原诱导的细胞免疫反应的体外免疫抑制和SJ-Crim的表达等方面对反映自然感染动物与高免动物免疫反应的MoAbs进行了表征，SJ-Crim是小鼠产生的多克隆抗SEA抗体的主要交叉反应独特型。日本血吸虫病。结果表明，对于与SEA结合的Ig G同型MoAbs，免疫抑制作用最强的抗SEA MoAbs表达SJ-Crim，来源于自然感染的小鼠。免疫印迹分析显示所有表达SJ-CRIM的抗SEA抗体均呈现相同的条带，但经弱高碘酸盐处理后条带消失。用不同的方法跨越异型屏障在MoAb上产生SJ-CRIM的表达表明，SJ-CRIM的表达是由生殖系基因编码的。这些数据表明，这种生殖系菌株间交叉反应独特型的表达与免疫抑制能力之间存在关联。此外，在免疫过程中发展起来的免疫调节网络。日本血吸虫感染是由在各种海洋中发现的一个碳水化合物表位(S)引起的。这些数据对交叉反应独特型作为血吸虫病血清学诊断工具的使用具有深远的意义。
A family of monoclonal antibodies (MoAb) was derived from the somatic cell fusion of P3NS1 myeloma cells and lymphoid cells from naturally infected mice or hyperimmunized mice. C57BL/6 mice were naturally infected withSchistosoma japonicum, and BALB/c mice were hyperimmunized with preparations ofS. japonicumsoluble egg antigens (SEA). The MoAbs which reflected the immune repertoire of naturally infected animals versus hyperimmune animals were characterized with regard to antibody isotype, antigen binding specificity, in‐vitro immunosuppression of antigen‐induced cell‐mediated immune responses and the expression of SJ‐CRIM, a major cross‐reactive idiotype which appears on polyclonal anti‐SEA antibodies generated during murineS. japonicuminfection. The data indicate that for MoAbs of the IgG isotype which bound SEA by ELISA, the most immunosuppressive anti‐SEA MoAbs identified expressed SJ‐CRIM and were derived from naturally infected mice. All anti‐SEA MoAbs expressing SJ‐CRIM showed an identical banding pattern on immunoblot analysis which was abrogated by weak periodate treatment. The generation of expression of SJ‐CRIM on MoAbs using differing methodologies across an allotype barrier indicates that the expression of SJ‐CRIMis encoded by a germline gene. These data indicate an association between expression of this germline interstrain cross‐reactive idiotype and immunosuppressive capacity. In addition, the immunoregulatory network which develops during immuneS. japonicuminfection is initiated by a carbohydrate epitope(s) found on various SEA. These data have profound implications in the use of the cross‐reactive idiotype as a serodiagnostic tool in schistosomiasis.