Widespread engraftment of neural progenitor and stem-like cells throughout the mouse brain.
Widespread engraftment of neural progenitor and stem-like cells throughout the mouse brain.
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DOI：
10.1016/s0041-1345(96)00163-7
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发表时间：
1997-02
影响因子：
0.9
通讯作者：
Rosanne M. Taylor;Evan Y. Snyder
中科院分区：
文献类型：
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作者：
Rosanne M. Taylor;Evan Y. Snyder
在所有1.5注射的小鼠中均发生NPC的有效植入。注射未引起显著的发病率或神经功能异常。在注射后的第一天，NPC定位于脑室下区和充满液体的脑空间。在第7天，发现标记的细胞广泛分散在整个CNS中，表明它们已从最初的植入部位迁移。NPC似乎并没有扰乱大脑的结构。这些细胞与宿主神经元和神经胶质混合在一起。事实上，NPC与宿主神经元和神经胶质之间的唯一区别是胞质和核周电子致密X-gal沉淀的存在。在注射后7至21天检查的小鼠冠状脑切片中，在89%中鉴定出标记的β 37.2细胞（图1）。尽管植入的模式和强度不同，但在脑的大多数区域都发现了NPC
RESULTSEfficient engraftment of NPCs occurred in all 1.5 injected mice. The injections did not cause significant morbidity or abnormal neurological function. In the first day after injection, the NPCs were localized in the subventricular zone and fluid-filled spaces of the brain. At 7 days, labeled cells were found widely dispersed throughout the CNS, suggesting they had migrated from the initial sites of engraftment. The NPC did not appear to disrupt the architecture of the brain. The cells mingled with host neurons and glia. In fact, the only distinction between NPCs and host neurons and glia was the presence of cytoplasmic and perinuclear electron-dense X-gal precipitates. Labeled (37.2 cells were identified in 89% of the coronal brain slices examined from mice 7 to 21 days after injection (Fig 1). NPCs were identified in most regions of the brain although the pattern and intensity of engraftment varied