ENHANCED CYTOCHROME-OXIDASE STAINING OF THE CUNEATE NUCLEUS IN THE RAT REVEALS A MODIFIABLE SOMATOTOPIC MAP
ENHANCED CYTOCHROME-OXIDASE STAINING OF THE CUNEATE NUCLEUS IN THE RAT REVEALS A MODIFIABLE SOMATOTOPIC MAP
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DOI：
10.1016/0006-8993(93)91642-6
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发表时间：
1993-05-28
期刊：
影响因子：
2.9
通讯作者：
EGGER, MD
中科院分区：
文献类型：
--
作者：
CROCKETT, DP;MASLANY, S;EGGER, MD
现有的细胞色素氧化酶（CO）染色技术进行了修改，以提高灵敏度和对比度，以检查模式的CO活性在成年Long-Evans大鼠背柱核（DCN）。在楔状核（CN）中间的喙尾侧有限区域内观察到强烈的CO活性的独特斑点。CO-染色最大分化约0.3-0.7毫米的奥贝斯尾部。在DCN的其他任何地方均未观察到CO斑点。跨神经节标记（WGA-HRP）证实，大鼠CN中的某些CO斑与前爪皮肤初级传入纤维的终末投射野有关。初级传入终止领域和CO染色模式的相应位置支持一个三方rostrocaudal司在大鼠CN，类似于其他研究人员所描述的猫，猴和浣熊。将CO染色模式与（1）细胞结构（Nissl染色切片）或（2）树突结构（微管相关蛋白2（MAP 2）的分布）或（3）逆行标记（WGA-HRP/HRP）楔丘脑细胞的组织进行比较，未发现与CO斑点相对应的地形学组织。出生后（至少产后11天）前爪传入神经切断或去除破坏了CN中的CO染色模式。
Existing cytochrome oxidase (CO)-staining techniques were modified to enhance sensitivity and contrast in order to examine patterns of CO-activity in the dorsal column nuclei (DCN) of adult Long-Evans rats. Within a rostrocaudally limited region in the middle of the cuneate nucleus (CN) distinctive blotches of intense CO-activity were observed. The CO-staining was maximally differentiated approximately 0.3-0.7 mm caudal to the obex. No CO-blotches were observed anywhere else in the DCN. Transganglionic labelling (WGA-HRP) demonstrated that some of the CO-blotches in the rat CN are related to the terminal projection fields of primary afferents from the skin of the forepaws. The corresponding location of primary afferent termination fields and CO-staining patterns supports a tripartite rostrocaudal division in the rat CN, similar to that described by other investigators in cats, monkeys and raccoons. Comparing the patterns of CO-staining to (1) the cytoarchitecture (Nissl-stained sections), or to (2) the dendritoarchitecture (distribution of microtubule-associated protein 2 (MAP2) or to (3) the organization of retrogradely labelled (WGA-HRP/HRP) cuneothalamic cells, revealed no topographical organization corresponding to the CO-blotches. Postnatal (at least up to 11 days postpartum) forepaw deafferentation or removal disrupted the CO-staining pattern in the CN.