Influence of oxygen saturation on the optical scattering properties of human red blood cells in the spectral range 250 to 2000 nm
Influence of oxygen saturation on the optical scattering properties of human red blood cells in the spectral range 250 to 2000 nm
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DOI：
10.1117/1.3127200
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发表时间：
2009-05-01
影响因子：
3.5
通讯作者：
Meinke, Martina
中科院分区：
文献类型：
--
作者：
Friebel, Moritz;Helfmann, Juergen;Meinke, Martina
测定了红细胞压积为33.2%的人红细胞悬液在250 ~ 2000 nm波长范围内（包括1100 nm以上）的吸收系数μ（a ′）、散射系数μ（s ′）、各向异性因子g（′）和有效散射系数μ（s）′，在文献中没有关于它的数据。结合逆蒙特卡罗模拟的光透射率和反射率的积分球测量进行了SAT O-2水平为100和0%。在高达1200 nm的波长范围内，吸收行为由血红蛋白吸收决定。高于细胞吸收的光谱范围显示出对SAT O-2没有依赖性，并且近似于水的吸收，其值比水的相应值低20%至30%。参数mu（s）和g受SAT O-2诱导的吸收变化的显著影响。在600 nm以上，μ（s）从85 mm（-1）的值连续下降到2000 nm处的30 mm（-1）的值。各向异性因子显示出随波长高于600 nm而略微减小。在水具有局部吸收最大值的1450和1900 nm的光谱区域中，g显示出显著降低至0.85，而μ（s）'增加。(C)2009年，由光学仪器工程师协会（Society of Photo-Optical Instrumentation Engineers）主办。[DOI：10.1117/1.3127200]
The intrinsic optical parameters absorption coefficient mu(a') scattering coefficient mu(s') anisotropy factor g(') and effective scattering coefficient mu(s)' were determined for human red blood cell (RBC) suspensions of hematocrit 33.2% dependent on the oxygen saturation (SAT O-2) in the wavelength range 250 to 2000 nm, including the range above 1100 nm, about which there are no data available in the literature. Integrating sphere measurements of light transmittance and reflectance in combination with inverse Monte Carlo simulation were carried out for SAT O-2 levels of 100 and 0%. In the wavelength range up to 1200 nm, the absorption behavior is determined by the hemoglobin absorption. The spectral range above the cells' absorption shows no dependence on SAT O-2 and approximates the absorption of water with values 20 to 30% below the respective values for water. Parameters mu(s) and g are significantly influenced by the SAT O-2-induced absorption changes. Above 600 nm, mu(s) decreases continuously from values of 85 mm(-1) to values of 30 mm(-1) at 2000 nm. The anisotropy factor shows a slight decrease with wavelengths above 600 nm. In the spectral regions of 1450 and 1900 nm where water has local absorption maxima, g shows a significant decrease down to 0.85, whereas mu(s)' increases. (C) 2009 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3127200]