Attomolar Protein Detection Using in-Hole Surface Plasmon Resonance
Attomolar Protein Detection Using in-Hole Surface Plasmon Resonance
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DOI：
10.1021/ja807704v
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发表时间：
2009-01-21
影响因子：
15
通讯作者：
Girotto, Emerson M.
中科院分区：
文献类型：
--
作者：
Ferreira, Jacqueline;Santos, Marcos J. L.;Girotto, Emerson M.
提出了一种孔内纳米孔表面等离子体共振传感方案。通过SiO(x)和金的薄层磨成的周期性纳米孔阵列被用来检测纳米孔内有机和生物分子的结合，同时阻挡孔外的金表面。这种新方法比以前的纳米孔阵列方法更有效，在纳米孔阵列方法中，响应与发生在孔内部和顶部金表面的结合事件有关。对结合事件的灵敏度提高和检测限降低与共振表面等离子体增强通过纳米孔阵列的传输有关。灵敏度为650nm/RIU，估计可检测3个原子分子的蛋白质。
An in-hole nanohole surface plasmon resonance sensing scheme is demonstrated. Arrays of periodic nanoholes milled through thin layers of SiO(x) and gold were used to detect the binding of organic and biological molecules inside the nanoholes, while blocking the gold surfaces outside the holes. This new approach is more efficient than the previous nanohole array method, where the response was related to binding events taking place inside of the holes and on the top gold surface. The improved sensitivity to binding events and lower detection limit are related to resonant surface plasmon enhanced transmission through the arrays of nanoholes. The sensitivity was found to be 650nm/RIU and the detection of three attomoles of proteins was estimated from this scheme.