INCIDENCE OF CHROMOSOME-ANOMALIES IN 1ST-CLEAVAGE MOUSE EMBRYOS OBTAINED FROM FROZEN-THAWED OOCYTES FERTILIZED INVITRO
INCIDENCE OF CHROMOSOME-ANOMALIES IN 1ST-CLEAVAGE MOUSE EMBRYOS OBTAINED FROM FROZEN-THAWED OOCYTES FERTILIZED INVITRO
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DOI：
10.1002/mrd.1120160303
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发表时间：
1987-03-01
期刊：
影响因子：
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通讯作者：
WHITTINGHAM, DG
中科院分区：
文献类型：
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作者：
GLENISTER, PH;WOOD, MJ;WHITTINGHAM, DG
为了评估低温保存对小鼠卵母细胞的影响，我们（1）检查了冷冻卵母细胞体外受精后移植到假孕寄养母体的胚胎的正常发育能力，（2）分析了第一次卵裂分裂时的染色体补体。冷冻卵母细胞的受精率低于对照卵母细胞（36% vs 66%），但胚胎移植后，两组中植入（67-68%）和形成正常胎儿（50-53%）的受精卵比例相似。冷冻不影响观察到的非整倍体发生率（1.5-3.3%）。冷冻卵母细胞产生的多倍体胚胎的频率几乎是对照组的两倍（15.8%对8.5%），但尚不清楚这是冷冻的真实的效应还是染色体制备技术产生的假象。
To assess the effect of low temperature storage on mouse oocytes we (1) examined the capacity for normal development of embryos derived from frozen oocytes fertilized in vitro after transfer to pseudopregnant foster mothers and (2) analyzed the chromosome complement at the first cleavage division. Fewer frozen than control oocytes were fertilized (36% vs 66%), but after embryo transfer the proportion of fertilized eggs that implanted (67-68%) and formed normal foetuses (50-53%) was similar in the two groups. Freezing did not affect the observed incidence of aneuploidy (1.5-3.3%). The frequency of polyploid embryos derived from frozen oocytes was almost double that of controls (15.8% vs 8.5%), but it is unclear whether this is a real effect of freezing or is an artifact produced by the chromosome preparation technique.