Induction of resistance to Toxoplasma gondii in human macrophages by soluble lymphocyte products.
Induction of resistance to Toxoplasma gondii in human macrophages by soluble lymphocyte products.
复制标题
通过可溶性淋巴细胞产物诱导人巨噬细胞对弓形虫的抵抗力。
作者：
S. Anderson;Simeon Bautista;J. Remington
采用放射自显影和光镜观察了不同条件下制备的淋巴细胞培养上清液对体外人单核细胞源性巨噬细胞内刚地弓形虫感染过程的影响。染色试验（DT）阳性受试者的淋巴细胞与刚地弓形虫裂解抗原（TLA）孵育，DT/阴性受试者的淋巴细胞与链激酶-链脲酶（SK-SD）孵育，或两种淋巴细胞与康那蛋白A （Con A）孵育得到的上清液能够激活巨噬细胞抑制或杀死细胞内的刚地弓形虫。用目标生物的同源抗原（TLA）制备的上清液比用异源抗原（SK-SD）或有丝分裂原（Con a）制备的上清液对巨噬细胞的弓形虫感染具有更强的抗性。淋巴细胞的数量是制备活性上清液的关键。提示可溶性淋巴细胞介质可激活人巨噬细胞体外抑制或杀伤弓形虫。
Autoradiography and light microscopy were used to study the effects of lymphocyte culture supernatants, prepared under a variety of conditions, on the course of intracellular Toxoplasma gondii infection in human monocyte-derived macrophages in vitro. Supernatants prepared by incubating lymphocytes of dye test- (DT) positive subjects with T. gondii lysate antigen (TLA), lymphocytes of DT/negative subjects with streptokinase-streptodornase (SK-SD), or both populations of lymphocytes with concanavalin A (Con A) were capable of activating macrophages to inhibit or kill intracellular T. gondii. Supernatants prepared with the homologous antigen (TLA) to the target organism appeared more active in conferring resistance to infection with T. gondii on macrophages than those prepared with a heterologous antigen (SK-SD) or mitogen (Con A). The number of lymphocytes was critical in preparing active supernatants. These results suggest that soluble lymphocyte mediators can activate human macrophages in vitro to inhibit or kill T. gondii.