Trimodal Gadolinium-Gold Microcapsules Containing Pancreatic Islet Cells Restore Normoglycemia in Diabetic Mice and Can Be Tracked by Using US, CT, and Positive-Contrast MR Imaging
Trimodal Gadolinium-Gold Microcapsules Containing Pancreatic Islet Cells Restore Normoglycemia in Diabetic Mice and Can Be Tracked by Using US, CT, and Positive-Contrast MR Imaging
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DOI：
10.1148/radiol.11101608
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发表时间：
2011-09-01
期刊：
影响因子：
19.7
通讯作者：
Bulte, Jeff W. M.
中科院分区：
文献类型：
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作者：
Arifin, Dian R.;Long, Christopher M.;Bulte, Jeff W. M.
目的：研制用于治疗I型糖尿病的免疫保护胰岛细胞的微囊，并对移植的胰岛细胞进行多模式细胞成像。材料和方法：所有动物实验均经机构动物护理和使用委员会批准。以硫酸鱼精蛋白作为临床级海藻酸交联剂，将DTPA功能化的金纳米颗粒与胰岛细胞共包裹。以常规的多L赖氨酸交联微囊和无囊化胰岛为对照。对7只链脲佐菌素诱导的糖尿病小鼠(n=7)和8只不植入胰岛细胞(n=8)的糖尿病小鼠进行了6周的体内胰岛素(C-肽)分泌监测，并对胰岛细胞的活性和葡萄糖反应性进行了评估。5只非糖尿病小鼠作为对照。样本之间的差异用非参数Wilcoxon Mann-Whitney方法计算。为了针对多次比较进行调整，P的显著水平
Purpose: To develop microcapsules that immunoprotect pancreatic islet cells for treatment of type I diabetes and enable multimodal cellular imaging of transplanted islet cells.Materials and Methods: All animal experiments were approved by the institutional animal care and use committee. Gold nanoparticles functionalized with DTDTPA (dithiolated diethylenetriaminepentaacetic acid): gadolinium chelates (GG) were coencapsulated with pancreatic islet cells by using protamine sulfate as a clinical-grade alginate cross linker. Conventional poly-L-lysine- cross-linked microcapsules and unencapsulated islets were included as controls. The viability and glucose responsiveness of islet cells were assessed in vitro, and in vivo insulin (C-peptide) secretion was monitored for 6 weeks in (streptozotocin-induced) diabetic mice with (n = 7) or without (n = 8) intraabdominally engrafted islet cells. Five nondiabetic mice were included as controls. Differences between samples were calculated by using a nonparametric Wilcoxon Mann-Whitney method. To adjust for multiple comparisons, a significance level of P