Ghrelin produces antidepressant-like effect in the estrogen deficient mice.
Ghrelin produces antidepressant-like effect in the estrogen deficient mice.
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生长素释放肽在雌激素缺乏的小鼠中产生抗抑郁样作用
DOI：
10.18632/oncotarget.19768
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发表时间：
2017-08-29
期刊：
影响因子：
--
通讯作者：
Cui RJ
中科院分区：
文献类型：
--
作者：
Fan J;Li BJ;Wang XF;Zhong LL;Cui RJ
最近的证据表明，胃饥饿素在抑郁症中起着重要作用。然而，在去卵巢小鼠中，胃饥饿素是否产生类似抗抑郁药的作用尚不清楚。本研究旨在探讨胃饥饿素对去卵巢小鼠的抗抑郁作用。在强迫游泳试验中，胃饥饿素显著缩短了静止时间，逆转了在去卵巢小鼠中观察到的“抑郁样”效应，而这种效应被他莫昔芬逆转。此外，免疫组织化学研究表明，ghrelin治疗逆转了卵巢切除术引起的c-Fos表达降低。雌激素拮抗剂他莫昔芬也能拮抗胃饥饿素对c-Fos表达的影响。此外，免疫印迹结果显示，胃饥饿素对海马脑源性神经营养因子（BDNF）有影响，而对额叶皮质磷酸化cAMP反应元件结合蛋白(pCREB)/CREB无影响。Ghrelin处理显著增加BrdU表达。因此，这些研究结果表明，胃饥饿素在去卵巢小鼠中产生抗抑郁样作用，雌激素受体可能参与了胃饥饿素的抗抑郁样作用。
Recent evidence shows that ghrelin plays an important role in depression. However, it was little known whether ghrelin produces antidepressant-like effect in the ovariectomized mice. The present study was aimed to investigate the antidepressant-like effects of the ghrelin in ovariectomized mice. In the forced swim test, ghrelin significantly decreased immobility time, reversing the “depressive-like” effect observed in ovariectomized mice, and this effect was reversed by the tamoxifen. In addition, immunohistochemical study indicated that ghrelin treatment reversed the reductions in c-Fos expression induced by ovariectomy. An estrogen antagonist tamoxifen also antagonized the effect of ghrelin on the c-Fos expression. Furthermore, the western blotting indicated that brain-derived neurotrophic factor (BDNF) in the hippocampus, but not phosphorylated cAMP response element-binding protein (pCREB)/CREB in the frontal cortex, were affected by ghrelin treatment. Ghrelin treatment significantly increased BrdU expression. Therefore, these findings suggest that ghrelin produces antidepressant-like effects in ovariectomized mice, and estrogen receptor may be involved in the antidepressant-like effects of the ghrelin.