Local gene transduction of cyclooxygenase-1 increases blood flow in injured atherosclerotic rabbit arteries
Local gene transduction of cyclooxygenase-1 increases blood flow in injured atherosclerotic rabbit arteries
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DOI：
10.1161/01.cir.0000158480.28733.89
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发表时间：
2005-04-12
期刊：
影响因子：
37.8
通讯作者：
Zoldhelyi, P
中科院分区：
文献类型：
--
作者：
Liu, Q;Chen, ZQ;Zoldhelyi, P
环氧化酶-1（cyclooxygenase-1，考克斯-1）是前列环素（prostacyclin，PGI（2））合成的限速成分，PGI（2）是一种重要的血管扩张剂和抗血栓分子。在球囊损伤、无动脉粥样硬化的猪动脉中，考克斯-1基因转导增加PGI（2）的产生，诱导持久的血管舒张，并减少血栓形成。我们测试的有效性考克斯-1局部基因转导的预防血管成形术后再狭窄的动脉粥样硬化动脉在hypercholesterolemic兔model.Methods和结果，我们在43渡边遗传性hypercholesteremic兔1颈动脉损伤和进行局部基因转导使用病毒载体含有考克斯-1基因（Ad考克斯-1，n=22）或无基因（Adnull，n=21）。3天后，AdCOX-1处理的动脉在花生四烯酸刺激下产生的PGI增加了100%（P
Background-Cyclooxygenase-1 (COX-1) is the rate-limiting component in the synthesis of prostacyclin (PGI(2)), an important vasodilator and antithrombotic molecule. In balloon-injured, atherosclerosis-free porcine arteries, COX-1 gene transduction increases PGI(2) production, induces durable vasodilation, and reduces thrombus formation. We tested the effectiveness of COX-1 local gene transduction for the prevention of postangioplasty restenosis in atherosclerotic arteries in a hypercholesterolemic rabbit model.Methods and Results-We injured 1 carotid artery in 43 Watanabe heritable hyperlipidemic rabbits and performed local gene transduction using a viral vector containing the COX-1 gene (AdCOX-1, n=22) or no genes (Adnull, n=21). Three days later, AdCOX-1-treated arteries stimulated with arachidonic acid produced 100% more PGI(2) (P