The distribution of E-cadherin during Xenopus laevis development.
The distribution of E-cadherin during Xenopus laevis development.
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DOI：
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发表时间：
1991
期刊：
影响因子：
4.6
通讯作者：
G. Levi;B. Gumbiner;J. Thiery
中科院分区：
文献类型：
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作者：
G. Levi;B. Gumbiner;J. Thiery
大量的实验证据表明，参与细胞与细胞和/或细胞与基质相互作用的细胞表面分子参与控制形态发生中的基本事件。E-钙粘蛋白是一种细胞粘附分子，直接参与控制上皮细胞之间的Ca 2（+）依赖性相互作用。我们在这里报告的E-钙粘蛋白的表达模式在非洲爪蟾的发育阶段，从早期胚胎到成人使用免疫荧光显微镜。虽然它的分布与鸡中的L-CAM和小鼠中的E-cadherin/Uvomorulin的分布有一些相似之处，但E-cadherin在非洲爪蟾中的分布呈现出一些特殊和独特的特征。在非洲爪蟾发育的早期阶段，E-钙粘蛋白不表达。该分子首先在晚期原肠胚（13-13.5 NF期）的外胚层中检测到。此时，非神经外胚层的外部和感觉层都积累了高水平的E-钙粘蛋白，而覆盖神经板的外胚层和尚未被原肠胚形成运动内化的内卷边缘区（IMZ）的区域是E-钙粘蛋白阴性的。与大多数其他物种不同，内胚层细胞不表达或表达极低水平的E-钙粘蛋白，直至20级NF。内胚层细胞只有在肠内形成分化良好的上皮细胞时才呈强E-钙粘蛋白阳性。在早期发育过程中，中胚层结构没有染色。在基板，在其他物种相比，E-钙粘蛋白消失后基板增厚非常迅速。在进一步的胚胎发育过程中，E-钙粘蛋白存在于皮肤、肠上皮、胰腺、许多单层上皮和大多数腺体中。肝细胞染色较弱，而包括原肾在内的大多数其他组织均为阴性。在中肾，Wolffian管和一些小管呈阳性。在变态过程中，在甲状腺激素的控制下，身体计划发生了深刻的重组，这涉及到皮肤和肠道等几种组织的退化和随后的再生。所有新形成的上皮表达高水平的E-钙粘蛋白。令人惊讶的是，皮肤和肠的退化上皮细胞即使在开始变得紊乱和退化之后也保持高水平的蛋白质。在成人中，染色在皮肤、腺体、肺、肠上皮和胰腺中较强，在肝脏中较弱，并且在大多数其他组织中不存在。我们的研究结果表明，在非洲爪蟾的E-钙粘蛋白的表达是强烈相关的分化上皮细胞的外观。
A vast amount of experimental evidence suggests that cell surface molecules involved in cell-to-cell and/or cell-to-substrate interactions participate in the control of basic events in morphogenesis. E-cadherin is a cell adhesion molecule directly implicated in the control of Ca2(+)-dependent interactions between epithelial cells. We report here the patterns of expression of E-cadherin in developmental stages of Xenopus laevis ranging from early embryo to adult using immunofluorescence microscopy. Although its distribution shares some similarities with those of L-CAM in the chicken and E-cadherin/Uvomorulin in the mouse, the distribution of E-cadherin in Xenopus presents several peculiar and unique features. In early stages of Xenopus development, E-cadherin is not expressed. The molecule is first detectable in the ectoderm of late gastrulas (stage 13-13.5 NF). At this time both the external and the sensory layer of the nonneural ectoderm accumulate high levels of E-cadherin while the ectoderm overlying the neural plate and regions of the involuting marginal zone (IMZ) not yet internalized by the movements of gastrulation are E-cadherin-negative. Unlike most other species, endodermal cells express no or very low levels of E-cadherin up to stage 20 NF. Endodermal cells become strongly E-cadherin-positive only when a well-differentiated epithelium forms in the gut. No mesodermal structures are stained during early development. In the placodes, in contrast to other species, E-cadherin disappears very rapidly after placode thickening. During further embryonic development E-cadherin is present in the skin, the gut epithelium, the pancreas, many monostratified epithelia and most glands. Hepatocytes are stained weakly while most other tissues, including the pronephros, are negative. In the mesonephros, the Wolffian duct and some tubules are positive. During metamorphosis a profound restructuring of the body plan takes place under the control of thyroid hormones, which involves the degeneration and subsequent regeneration of several tissues such as the skin and the gut. All newly formed epithelia express high levels of E-cadherin. Surprisingly, degenerating epithelia of both skin and intestine maintain high levels of the protein even after starting to become disorganized and to degenerate. In the adult, staining is strong in the skin, the glands, the lungs, the gut epithelium and the pancreas, weak in the liver and absent from most other tissues. Our results show that the expression of E-cadherin in Xenopus is strongly correlated with the appearance of differentiated epithelia.