Effects of 9-aminoacridine on bacteriophage T4 deoxyribonucleic acid synthesis.
Effects of 9-aminoacridine on bacteriophage T4 deoxyribonucleic acid synthesis.
复制标题
9-氨基吖啶对噬菌体T4脱氧核糖核酸合成的影响。
DOI：
10.1016/0022-2836(70)90191-9
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发表时间：
1970
影响因子：
5.6
通讯作者：
L. Lerman
中科院分区：
文献类型：
--
作者：
S. Altman;L. Lerman
当9-氨基吖啶被T_4噬菌体感染的大肠杆菌培养基中含有时，通过各种沉降研究，观察到对放射性标记的细胞内噬菌体DNA命运的各种影响，成熟噬菌体颗粒和DNA复制中间形式的产生速率比DNA合成的总速率受到更严重的影响。9-氨基吖啶干扰复制过程的正常功能，产生大量新合成的小T4 DNA片段，并似乎阻止亲本DNA从复制复合物中释放。感染噬菌体中9-氨基吖啶抗性标记的存在缓解了最终包装步骤的抑制，部分缓解了早期复制步骤的故障。在感染后20分钟，从培养基中除去病毒的大小（最佳浓度）。当9-氨基吖啶存在时产生的小DNA片段的大小取决于药物的浓度，并且随着开发的进展，后面的片段更接近噬菌体DNA的大小。当9-aminoacridine从培养基中去除时，部分DNA似乎被用于随后的复制。9-aminoacridine对噬菌体DNA包装的影响与细胞内正常复制DNA的产生不同。
When 9-aminoacridine is included in the culture medium ofEscherichia coliinfected with bacteriophage T4, various effects upon the fate of radioactively labeled intracellular phage DNA as examined by various sedimentation studies are observed.The rates of production of mature phage particles and a replicative intermediate form of DNA are much more severely affected than the gross rate of DNA synthesis. 9-Aminoacridine disturbs the normal function of the replicative process to yield large amounts of newly synthesized, small T4 DNA fragments and appears to block release of parental DNA from the replicative complex. The presence of a 9-aminoacridine resistance marker in the infecting phage relieves the inhibition both of the final packaging step, and in part, the malfunctioning of the earlier replicative steps.DNA synthesis is necessary for production of viable phage after 9-aminoacridine (at optimum concentration) is removed from the culture medium at 20 minutes after infection.The size, of the small DNA fragments produced when 9-aminoacridine is present is dependent upon the concentration of the drug and as development progresses, the later fragments more nearly approach the size of phage DNA. Part of this DNA appears to be utilized in subsequent replication when 9-aminoacridine is removed from the culture medium.The effect of 9-aminoacridine upon phage DNA packaging is distinct from that upon the production of normal replicating DNA within the cell.