Interaction of methylmercury chloride with cellular energetics and related processes.
Interaction of methylmercury chloride with cellular energetics and related processes.
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氯化甲基汞与细胞能量学和相关过程的相互作用。
DOI：
10.1016/0041-008x(84)90001-2
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发表时间：
1984
影响因子：
3.8
通讯作者：
Donald R. Miller
中科院分区：
文献类型：
--
作者：
Ariff I. Ally;Jenny Phipps;Donald R. Miller
暴露于氯化甲基汞后，酿酒酵母的全细胞氧摄取停止。在可发酵的碳源上，二氧化碳在呼吸停止后继续释放，表明发酵仍然活跃。葡萄糖和甘油的摄取也会持续到各自的过程，发酵和呼吸，被完全抑制。蛋白质和核酸的合成被类似浓度的甲基汞阻断，而细胞色素c，电子传递链的末端成分，不受毒物的影响。令人惊讶的是，细胞内ATP在处理的细胞中比在对照中更高，尽管它们最终响应于更高的浓度或更长的暴露而下降。高压液相色谱分析表明，其他核苷酸的量要么不变，要么增加。整个抑制过程是可逆的，随着时间的推移或新鲜介质在低甲基汞浓度。这些结果不支持由几位作者所表达的由膜扰动引起的ATP生物合成抑制的假设。这些数据表明，ATP的减少，当诱导的有机汞，是一个次要的过程，而不是直接的线粒体毒性的结果。
Upon exposure to methylmercury chloride, the whole-cell oxygen uptake by the yeast Saccharomyces cerevisiae ceases. On a fermentable carbon source, carbon dioxide continues to be evolved after respiration has stopped, indicating that fermentation is still active. Dextrose and glycerol uptake also persists until the respective processes, fermentation and respiration, are totally inhibited. Protein and nucleic acid synthesis are blocked with similar concentrations of methylmercury, while cytochrome c, the terminal component of the electron transport chain, is unaltered by the toxicant. Surprisingly, the intracellular ATP is higher in the treated cells than in the controls, although they eventually fall in response to higher concentration or longer exposure. High-pressure liquid chromatography profiles show that the amounts of the other nucleotides are either unaltered or increased. The entire inhibitory process is reversible with time or fresh medium at low methylmercury concentrations. These results do not support the hypothesis expressed by several authors of an inhibition of ATP biosynthesis resulting from membrane perturbation. These data suggest that the decrease in ATP—when induced by the organomercurial—is a secondary process and is not the result of direct mitochondrial toxicity.