The Bacillus subtilis acyl lipid desaturase is a Δ5 desaturase
The Bacillus subtilis acyl lipid desaturase is a Δ5 desaturase
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DOI：
10.1128/jb.185.10.3228-3231.2003
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发表时间：
2003-05-01
影响因子：
3.2
通讯作者：
de Mendoza, D
中科院分区：
文献类型：
--
作者：
Altabe, SG;Aguilar, P;de Mendoza, D
最近报道枯草芽孢杆菌（Bacillus subtilis）合成在位置Δ 5、Δ 7和Δ 9处具有双键的不饱和脂肪酸（UFA）（M. H.韦伯，W。克莱因湖Muller，U. M. Niess和M. A.马拉希埃尔山Microbiol. 39：1321-1329，2001）。因为这一发现对于B所显示的双键位置特异性具有相当重要的意义。枯草酰基脂质去饱和酶，我们试图证实这一观察。我们报道了由B合成的不饱和脂肪酸的双键。枯草芽孢杆菌的脂肪酸链仅位于Δ 5位置，而与生长温度和脂肪酸链的长度无关。
Bacillus subtilis was recently reported to synthesize unsaturated fatty acids (UFAs) with a double bond at positions, Delta5, Delta7, and Delta9 (M. H. Weber, W. Klein, L. Muller, U. M. Niess, and M. A. Marahiel, Mol. Microbiol. 39:1321-1329, 2001). Since this finding would have considerable importance in the double-bond positional specificity displayed by the B. subtilis acyl lipid desaturase, we have attempted to confirm this observation. We report that the double bond of UFAs synthesized by B. subtilis is located exclusively at the Delta5 position, regardless of the growth temperature and the length chain of the fatty acids.