Ketone body production in diabetic ketosis by other than liver.
Ketone body production in diabetic ketosis by other than liver.
复制标题
糖尿病酮症中酮体的产生除了肝脏之外。
DOI：
10.1016/0026-0495(83)90144-0
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Landau,BR
中科院分区：
文献类型：
--
作者：
Scofield,RF;Schumann,WC;Kumaran,K;Landau,BR
为了确定酮体（由肝脏以外的组织由脂肪酸合成）是否进入循环，给糖尿病酮症大鼠注射[6，13 - 14 C]棕榈酸钠。从每只大鼠排泄的尿液和其尸体的水提取物中分离出羟基丁酸酯。提取物中14 C在羟基丁酸四个碳上的分布与尿液中相同。羟基丁酸酯的碳1与碳3中的14 C比平均为1.80，碳2与碳4中的14 C比平均为1.31。当由灌注的肝脏形成时，羟基丁酸具有与碳2与碳4的比率相同的碳1与碳3的比率。结果表明，由肝脏以外的组织合成的羟基丁酸酯在循环中与肝脏合成的羟基丁酸酯混合，然后一部分混合物随尿液排出。碳1与碳3的比率3和碳2与碳4的比率之间的差异计算为具有肝脏以外的组织作为其来源的酮症糖尿病大鼠的循环中羟基丁酸的估计最小值15%至17%。假设肝脏和肾脏是糖尿病酮症中酮体的来源，肾脏产生的酮体在没有首先进入循环之前不会排泄到尿液中。
To determine if ketone bodies, synthesized from fatty acids by tissues other than the liver, enter the circulation, rats in diabetic ketosis were injected with sodium [6,13-14C]palmitate. Hydroxybutyrate was isolated from the urine excreted by each rat and from an aqueous extract of its carcass. The distribution of14C in the four carbons of hydroxybutyrate in the extract was the same as in the urine. The ratio of14C in carbon 1 to carbon 3 of the hydroxybutyrate averaged 1.80 and averaged 1.31 in carbon 2 to carbon 4. Hydroxybutyrate when formed by perfused liver has the same carbon 1-to-carbon 3 ratio as carbon 2-to-carbon 4 ratio. The results indicate that hydroxybutyrate synthesized by tissues other than the liver mixes in the circulation with that synthesized by the liver and a portion of the mix is then excreted in the urine. The difference between the carbon 1-to-3 carbon ratio 3 and carbon 2-to-carbon 4 ratio calculates to an estimated minimum of 15% to 17% of the hydroxybutyrate in the circulation of the ketotic diabetic rat having tissues other than the liver as its source. Assuming the liver and kidneys are the sources of the ketone bodies in diabetic ketosis, the ketone bodies produced by the kidneys are not excreted into the urine without first entering the circulation.