An interbacterial DNA deaminase toxin directly mutagenizes surviving target populations.
An interbacterial DNA deaminase toxin directly mutagenizes surviving target populations.
复制标题
细菌间DNA脱氨酶毒素直接诱变存活的目标群体。
DOI：
10.7554/elife.62967
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发表时间：
2021-01-15
期刊：
影响因子：
7.7
通讯作者：
Mougous JD
中科院分区：
文献类型：
--
作者：
de Moraes MH;Hsu F;Huang D;Bosch DE;Zeng J;Radey MC;Simon N;Ledvina HE;Frick JP;Wiggins PA;Peterson SB;Mougous JD
当细菌细胞接触时，由毒素传递介导的拮抗作用经常发生。这种接触对受体细胞产生长期有益影响的可能性尚未研究。在这里，我们研究了中毒的影响，通过六型分泌系统（T6SS）的伯克霍尔德氏菌cenocepacia的胞嘧啶脱氨酶DddA。尽管它具有杀伤潜力，但我们观察到几种细菌物种对DddA有抵抗力，而是积累突变。这些突变可导致抗生素耐药性的获得，表明即使在没有杀死的情况下，细菌间的拮抗作用也会对目标群体产生深远的影响。对来自脱氨酶超家族的其他毒素的研究表明，致突变活性是这些蛋白质的共同特征，包括我们显示的靶向单链DNA的代表性蛋白质，并显示出明显不同的结构。我们的研究结果表明，细菌之间的拮抗作用的一个令人惊讶的结果可能是通过直接致突变毒素的作用促进适应。
When bacterial cells come in contact, antagonism mediated by the delivery of toxins frequently ensues. The potential for such encounters to have long-term beneficial consequences in recipient cells has not been investigated. Here, we examined the effects of intoxication by DddA, a cytosine deaminase delivered via the type VI secretion system (T6SS) of Burkholderia cenocepacia. Despite its killing potential, we observed that several bacterial species resist DddA and instead accumulate mutations. These mutations can lead to the acquisition of antibiotic resistance, indicating that even in the absence of killing, interbacterial antagonism can have profound consequences on target populations. Investigation of additional toxins from the deaminase superfamily revealed that mutagenic activity is a common feature of these proteins, including a representative we show targets single-stranded DNA and displays a markedly divergent structure. Our findings suggest that a surprising consequence of antagonistic interactions between bacteria could be the promotion of adaptation via the action of directly mutagenic toxins.