Neurexin Restricts Axonal Branching in Columns by Promoting Ephrin Clustering
Neurexin Restricts Axonal Branching in Columns by Promoting Ephrin Clustering
复制标题
Neurexin 通过促进 Ephrin 聚类来限制柱中的轴突分支
DOI：
10.1016/j.devcel.2017.03.004
复制
发表时间：
2017-04-10
影响因子：
11.8
通讯作者：
Han, Junhai
中科院分区：
文献类型：
--
作者：
Liu, Lina;Tian, Yao;Han, Junhai
在脊椎动物和无脊椎动物的神经系统中，轴突的柱状限制对于形成非重叠的感受野和保存来自外周的空间感觉信息是至关重要的，但其潜在的分子机制在很大程度上仍然未知。在这里，我们表明，果蝇同源的α-neurexin（DNS 10）在L4轴突分支的柱状限制中起着至关重要的作用。从L4神经元的DNP 3的耗尽导致L4轴突分支到相邻列的错误投射，由于受损的肝配蛋白聚集。合适的肝配蛋白簇需要其与DNF 3的细胞内区域的相互作用。此外，我们还发现Tm 2神经元中的果蝇神经配素4（DNlg 4）与DNlg结合，并启动L4神经元中的DNlg聚集，随后诱导ephrin聚集。我们的研究表明，DNP 4促进ephrin集群，并揭示了ephrin/Eph信号从相邻的L4神经元限制轴突分支的L4神经元列。
Columnar restriction of neurites is critical for forming nonoverlapping receptive fields and preserving spatial sensory information from the periphery in both vertebrate and invertebrate nervous systems, but the underlying molecular mechanisms remain largely unknown. Here, we demonstrate that Drosophila homolog of alpha-neurexin (DNrx) plays an essential role in columnar restriction during L4 axon branching. Depletion of DNrx from L4 neurons resulted in misprojection of L4 axonal branches into neighboring columns due to impaired ephrin clustering. The proper ephrin clustering requires its interaction with the intracellular region of DNrx. Furthermore, we find that Drosophila neuroligin 4 (DNlg4) in Tm2 neurons binds to DNrx and initiates DNrx clustering in L4 neurons, which subsequently induces ephrin clustering. Our study demonstrates that DNrx promotes ephrin clustering and reveals that ephrin/Eph signaling from adjacent L4 neurons restricts axonal branches of L4 neurons in columns.