Examination of the role of FMRFamide-related peptides in the circadian clock of the cockroach Leucophaea maderae
Examination of the role of FMRFamide-related peptides in the circadian clock of the cockroach Leucophaea maderae
复制标题
检查 FMRFamide 相关肽在蟑螂 Leucophaea maderae 生物钟中的作用
DOI：
10.1007/s00441-008-0585-9
复制
发表时间：
2008
影响因子：
3.6
通讯作者：
M. Stengl
中科院分区：
文献类型：
--
作者：
Sandra Soehler;S. Neupert;R. Predel;M. Stengl
副髓质是蟑螂的生物钟，富含神经肽。在这些神经肽中有FMRFamide相关肽（FaRP），其通常共享C-末端RFamide。作为第一步了解的功能作用的FaRPs的昼夜节律钟的蟑螂，免疫细胞化学与抗血清对各种FaRPs，MALDI-TOF质谱，并注射两个FaRPs结合转轮测定进行。在与副髓质相关的最大四个索马簇和前脑的大多数神经髓鞘中发现了显著的FMRFamide样免疫反应性。通过MALDI-TOF质谱分析，得到了蟑螂的各种扩展FMRFamides。maderae在胸周对称器官中被部分鉴定，这些器官是已知在昆虫中积累扩展FMRFamides的结构。通过质量匹配，在副髓质中也检测到这些肽中的几种。注射FMRFamide和Pea-FMRFa-7（DRSDNFIRF-NH 2）到副髓质附近引起运动活动节律在昼夜节律时间8，18和4的时间依赖性相移。因此，我们的数据表明，不同的FaRPs在控制昼夜运动活动节律的作用。马德拉酒
The accessory medulla, the circadian clock of the cockroachLeucophaea maderae, is abundant in neuropeptides. Among these neuropeptides are the FMRFamide-related peptides (FaRPs), which generally share the C-terminal RFamide. As a first step toward understanding the functional role of FaRPs in the circadian clock of the cockroach, immunocytochemistry with antisera against various FaRPs, MALDI-TOF mass spectrometry, and injections of two FaRPs combined with running-wheel assays were performed. Prominent FMRFamide-like immunoreactivity was found in maximally four soma clusters associated with the accessory medulla and in most neuropils of the protocerebrum. By MALDI-TOF mass spectrometry, various extended FMRFamides of the cockroachL. maderaewere partially identified in thoracic perisympathetic organs, structures known to accumulate extended FMRFamides in insects. By mass match, several of these peptides were also detected in the accessory medulla. Injections of FMRFamide and Pea-FMRFa-7 (DRSDNFIRF-NH2) into the vicinity of the accessory medulla caused time-dependent phase-shifts of locomotor activity rhythms at circadian times 8, 18, and 4. Thus, our data suggest a role for the different FaRPs in the control of circadian locomotor activity rhythms inL. maderae.