Cytochrome c-lipid interactions studied by resonance Raman and 31P NMR spectroscopy. Correlation between the conformational changes of the protein and the lipid bilayer.-猫眼课题宝

通过共振拉曼和 31P NMR 光谱研究细胞色素 c-脂质相互作用。

DOI：

10.1021/bi00101a025

发表时间：

1991

期刊：

Biochemistry

影响因子：

2.9

通讯作者：

中科院分区：

生物学3区

文献类型：

作者：

T. Heimburg;P. Hildebrandt;D. Marsh

用蛋白质血红素基的共振拉曼光谱和磷脂头基的~（31）P核磁共振研究了细胞色素c与带负电荷的脂质的相互作用。凝胶到流体相转变的二肉豆蔻酰磷脂酰甘油诱导的结合细胞色素c的构象和配位平衡的变化，记录的指纹和标记带区域的共振拉曼光谱。结合细胞色素的构象和协调位移也诱导上的二油酰甘油或二油酰磷脂酰胆碱与二油酰磷脂酰甘油的混合物。就二油酰甘油而言，即使含量低至5摩尔%，也会发生显着变化。结合细胞色素c诱导或增加近各向同性扩散脂质的31 P NMR光谱研究的不同脂质注册的内容。二油酰甘油的混合物也增加了二油酰磷脂酰甘油的双层曲率，在50摩尔%的浓度下诱导一个倒置的六边形相;脂质中自发曲率的趋势似乎放松了蛋白质中检测到的构象变化。

The interaction of cytochrome c with negatively charged lipids has been studied by resonance Raman spectroscopy of the protein heme group and 31P NMR of the phospholipid headgroups. The gel-to-fluid-phase transition of dimyristoylphosphatidylglycerol induces shifts in the conformational and coordination equilibria of the bound cytochrome c, as recorded by the resonance Raman spectra in the fingerprint and marker band regions. Conformational and coordination shifts of the bound cytochrome are also induced on admixture of dioleoylglycerol or dioleoylphosphatidylcholine with dioleoylphosphatidylglycerol. In the case of dioleoylglycerol, significant changes take place even at levels as low as 5 mol %. Binding of cytochrome c induces or increases the content of near isotropically diffusing lipid registered by the 31P NMR spectra of the different lipids studied. Admixture of dioleoylglycerol also increases the bilayer curvature of dioleoylphosphatidylglycerol, inducing an inverted hexagonal phase at 50 mol % concentration; the tendency to spontaneous curvature in the lipid appears to relax the conformational change detected in the protein.