SEM and TEM analysis of water degradation of human dentinal collagen
SEM and TEM analysis of water degradation of human dentinal collagen
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DOI：
10.1002/jbm.b.10560
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发表时间：
2003-07-15
影响因子：
3.4
通讯作者：
Oguchi, H
中科院分区：
文献类型：
--
作者：
Hashimoto, M;Tay, FR;Oguchi, H
最近，一些长期研究报告了基于粘合测试后断裂表面观察的胶原原纤维水解降解的证据。这些研究表明，粘合强度下降的原因之一是粘合内胶原原纤维的降解。然而，人们担心牙本质胶原纤维在不含口腔细菌或酶的水中可能是稳定的。因此，本研究旨在阐明裸露胶原纤维在水储存500天后的微形态变化。为了制备暴露的胶原原纤维，使用两种市售酸调节剂：All-Etch（10％磷酸）和Uni-Etch（32％磷酸）（Bisco，Inc.）对切片和抛光的人牙本质表面进行酸调节15秒。这些样本在37℃的蒸馏水中储存1天（对照），持续500天。储存期结束后，使用 SEM 和 TEM 检查样品。在SEM和TEM检查下，在水中放置500天后，发现标本发生了微形态变化（胶原网紊乱、原纤维间隙变宽、胶原原纤维直径变细）。 (C) 2003 年 Wiley 期刊公司。
Recently several long-term studies have reported evidence of the hydrolytic degradation of collagen fibrils based on fractured surface observations after bond testing. Those studies suggested that one cause of the decline in the bond strength was the degradation of the collagen fibrils within the bonds. However, one concern has been raised that the dentinal collagen fibrills may be stable in water that does not contain oral bacteria or enzymes. Therefore, the present study aimed to clarify the micromorphological change in naked collagen fibrils after 500 days of water storage. To prepare exposed collagen fibrils, sectioned and polished human dentin surfaces were acid conditioned for 15 s with the use of two commercially available acid conditioners: All-Etch (10% phosphoric acid) and Uni-Etch (32% phosphoric acid) (Bisco, Inc.). Those specimens were stored in distilled water at 37 degreesC for 1 day (control) for 500 days. After the storage periods, the samples were examined with the use of SEM and TEM. Under SEM and TEM examination, micromorphological alterations (disarrangement of collagen web, widening the interfibrillar space, and the thinning diameter of collagen fibrils) were found in the specimens after 500 days in water. (C) 2003 Wiley Periodicals, Inc.