Time-resolved fluorescence anisotropies of diphenylhexatriene and perylene in solvents and lipid bilayers obtained from multifrequency phase-modulation fluorometry.
Time-resolved fluorescence anisotropies of diphenylhexatriene and perylene in solvents and lipid bilayers obtained from multifrequency phase-modulation fluorometry.
复制标题
通过多频相位调制荧光测定法获得溶剂和脂质双层中二苯基己三烯和苝的时间分辨荧光各向异性。
DOI：
10.1021/bi00323a021
复制
发表时间：
1985
期刊：
影响因子：
2.9
通讯作者：
Maliwal,BP
中科院分区：
文献类型：
--
作者：
Lakowicz,JR;Cherek,H;Maliwal,BP
伊利诺伊大学厄巴纳-香槟分校物理系，1984年5月16日，伊利诺伊州厄巴纳，伊利诺伊州。摘要：荧光各向异性的时间分辨衰变是由偏振发射的平行和垂直分量之间的相角差和调制幅度的比率的频域测量得到的。这些数据是在1至200 MHz的调制频率下测量的。为了证明这种方法的普遍适用性，我们描述了各向异性的简单和复杂衰减的分辨率。特别是，我们解决了各向异性的一次、二次和三次指数衰减，以及脂双层内荧光团的受阻旋转运动。获得这些结果的简便性和快速性表明，频域测量对于确定各向异性的复杂衰变是实用和可靠的。
Department of Physics, University of Illinois at Urbana—Champaign, Urbana, Illinois 61801 Received May 16, 1984 abstract: Time-resolved decays of fluorescence anisotropy were obtained from frequency-domain mea-surements of the phase angle difference between the parallel and perpendicular components of the polarized emission and the ratio of the modulated amplitudes. These data were measured at modulation frequencies ranging from 1 to 200 MHz. To demonstrate the general applicability of this method, we describe the resolution of both simple and complex decays of anisotropy. In particular, we resolved single, double, and triple exponential decays of anisotropy and the hindered rotational motions of fluorophores within lipid bilayers. The ease and rapidity with which these results were obtained indicate that frequency-domain measurements are both practical and reliable for the determination of complex decays of anisotropy.