Transcriptome analysis provides novel insights into the function of PI3K/AKT pathway in maintaining metabolic homeostasis of Chinese perch muscle
Transcriptome analysis provides novel insights into the function of PI3K/AKT pathway in maintaining metabolic homeostasis of Chinese perch muscle
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DOI：
10.1016/j.aqrep.2021.100838
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发表时间：
2021-11
影响因子：
3.7
通讯作者：
Xin Zhu;Linrui Ren;Jingjie Liu;Lin Chen;Jia Hua Cheng;W. Chu;Jianshe Zhang
中科院分区：
文献类型：
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作者：
Xin Zhu;Linrui Ren;Jingjie Liu;Lin Chen;Jia Hua Cheng;W. Chu;Jianshe Zhang
骨骼肌在鱼的生命周期中表现出显著的动力性和可塑性。然而，鱼类骨骼肌代谢稳态动态调节的分子机制尚不清楚。PI3K/AKT信号在生物生长和关键细胞过程中通过调节生长因子信号在细胞生理学中发挥核心作用。为了揭示PI3K/AKT信号通路在河豚肌肉代谢调控中的作用，采用Illumina测序技术研究了PI3K抑制剂(LY294002)对肌肉转录组的影响。在对照组和LY294002处理组之间，共鉴定出619个差异表达基因(DEG)。功能富集分析表明，DEGS主要与代谢动态平衡有关，如氨基酸的生物合成、糖酵解/糖异生、甘油脂代谢过程等。组织切片分析显示，LY294002注射组肌纤维间隙增大，胞浆含量减少，符合肌肉萎缩的特点。这些结果表明，PI3K/AKT信号通路在维持鱼肌肉代谢平衡中起着关键作用，从而保证了肌肉的正常生理和生长。此外，本研究为进一步阐明鱼肌肉中PI3K/AKT信号代谢动态平衡的分子机制提供了方向。
Skeletal muscle exhibits significant dynamism and plasticity over the fish life cycle. However, the molecular mechanism of dynamic regulation of metabolic homeostasis in fish skeletal muscle remains unclear. PI3K/AKT signaling plays a central role in cellular physiology by mediating growth factor signals during organismal growth and critical cellular processes. In order to reveal the role of PI3K/AKT signaling pathway in metabolic regulation of Chinese perch muscle, Illumina sequencing technology was preformed to mine the alterations of muscle transcriptome under PI3K inhibitor (LY294002) treatment. Total 619 unigenes were identified as significantly differentially expressed genes (DEGs) between the control and LY294002 treatment groups. Functional enrichment analysis revealed that DEGs were mostly associated with metabolic homeostasis, such as Biosynthesis of amino acids, Glycolysis/gluconeogenesis, Glycerolipid metabolism process. Furthermore, the histological section analysis showed that the spaces between muscle fibers in LY294002 injection group were increased and the cytoplasmic contents were reduced, which was consistent with the characteristics of muscle atrophy. Together with the results, it was suggested that the PI3K/AKT signaling pathway plays a crucial role in maintaining the balance of metabolism in fish muscle, which in turn ensures normal muscle physiology and growth. In addition, this study could provide a direction for the future research of elucidating the molecular mechanisms of metabolic homeostasis of PI3K/AKT signaling in fish muscle.