The use of monoclonal antibodies to probe human apolipoprotein B structure and function.
The use of monoclonal antibodies to probe human apolipoprotein B structure and function.
复制标题
使用单克隆抗体探测人载脂蛋白 B 的结构和功能。
DOI：
10.1139/o85-112
复制
发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Y. Marcel
中科院分区：
文献类型：
--
作者：
R. Milne;Y. Marcel
载脂蛋白（apo）B在血浆脂质转运和胆固醇稳态维持中起重要作用。确定载脂蛋白B结构的尝试受到其化学和物理性质所造成的技术障碍的阻碍。近年来，抗人载脂蛋白B的单克隆抗体（Mab）被用作研究载脂蛋白B结构和异质性的探针。某些单抗能够阻断低密度脂蛋白（LDL）apo B与细胞表面LDL受体的结合，这可能反映了其抗原决定簇与受体识别结构域的接近。已在apo B的肝（apo B-100）和肠（apo B-48）形式上以及apo B有限蛋白水解产生的片段上研究了Mab识别的抗原决定簇的分布。一些单克隆抗体对载脂蛋白B-100具有特异性，而另一些单克隆抗体与载脂蛋白B-48交叉反应。与琼脂糖凝胶偶联的载脂蛋白B-100特异性单克隆抗体已用于分离肠源和肝源的含载脂蛋白B的脂蛋白，并测定了它们各自的脂质和载脂蛋白组成。使用分离的级分，已经表明apo B-100而不是apo B-48可以与LDL受体反应。大多数单克隆抗体不能与脱脂和再溶解的载脂蛋白B反应，但在某些情况下，如果溶解的载脂蛋白B重新掺入脂质囊泡中，免疫反应性可以恢复。这些实验表明，不同的决定因素有不同的脂质要求，他们的表达。（250字处删节）
Apolipoprotein (apo) B plays an important role in plasma lipid transport and in the maintenance of cholesterol homeostasis. Attempts to determine the structure of apo B have been hampered by technical obstacles resulting from its chemical and physical properties. Recently monoclonal antibodies (Mabs) against human apo B have been used as probes to study apo B structure and heterogeneity. Certain Mabs are capable of blocking binding of low density lipoprotein (LDL) apo B to the cell surface LDL receptor, which presumably reflects the proximity of their antigenic determinants to the receptor recognition domain. The distribution of antigenic determinants recognized by Mabs has been studied on the hepatic (apo B-100) and intestinal (apo B-48) forms of apo B and on fragments generated by limited proteolysis of apo B. Some Mabs are specific for apo B-100, whereas others cross-react with apo B-48. Apo B-100 specific Mabs coupled to Sepharose have been used to isolate separately apo-B-containing lipoproteins of intestinal and hepatic origin and their respective lipid and apolipoprotein compositions have been determined. Using the separated fractions it has been shown that apo B-100, but not apo B-48, can react with the LDL receptor. Most Mabs failed to react with apo B which had been delipidated and resolubilized, but in some cases immunoreactivity could be recovered if the solubilized apo B were reincorporated into lipid vesicles. These experiments showed that different determinants had different lipid requirements for their expression.(ABSTRACT TRUNCATED AT 250 WORDS)