Isolation and Visualization of Gliding Motility Machinery in Bacteroidota
Isolation and Visualization of Gliding Motility Machinery in Bacteroidota
复制标题
拟杆菌滑动运动机械的分离和可视化
DOI：
10.1007/978-1-0716-3060-0_21
复制
发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Nakane Daisuke
中科院分区：
文献类型：
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作者：
Shibata Satoshi;Nakane Daisuke
拟杆菌门（以前称为拟杆菌门）的许多成员能附着在固体表面上并在其上移动.这种类型的细菌运动被称为滑行，不涉及传统的细菌运动机制，如鞭毛和皮利。为了解土壤细菌黄杆菌（Flavobacterium johnsoniae）和海洋细菌大腐螺旋菌（Saprospira grandis）等拟杆菌的滑行运动机制，采用负染电镜技术对这两种细菌的滑行运动机制进行了研究。在这里，我们描述的方法，直接观察滑动运动机制在拟杆菌的透射电子显微镜。
Many members of the phylumBacteroidota(formerly calledBacteroidetes) adhere to and move on solid surfaces. This type of bacterial motility is called gliding and does not involve the conventional bacterial motility machinery, such as flagella and pili. To understand the mechanism of gliding motility of someBacteroidotabacteria such as a soil bacteriumFlavobacterium johnsoniaeand a marine bacteriumSaprospira grandis, the gliding motility machines of these two bacteria have been analyzed by electron microscopy with negative staining. Here, we describe methods to directly observe the gliding motility machinery inBacteroidotaby transmission electron microscopy.