Normal patterns of expression of glycosylphosphatidylinositol-anchored proteins on different subsets of peripheral blood cells:: A frame of reference for the diagnosis of paroxysmal nocturnal hemoglobinuria
Normal patterns of expression of glycosylphosphatidylinositol-anchored proteins on different subsets of peripheral blood cells:: A frame of reference for the diagnosis of paroxysmal nocturnal hemoglobinuria
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DOI：
10.1002/cyto.b.20087
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发表时间：
2006-03-01
影响因子：
3.4
通讯作者：
Orfao, A
中科院分区：
文献类型：
--
作者：
Hernández-Campo, PM;Almeida, J;Orfao, A
背景资料：评价糖基磷脂酰肌醇锚定膜蛋白（GPI-AP）的表达目前用于诊断阵发性睡眠性血红蛋白尿症（PNH）。本研究分析了正常人外周血中不同造血细胞亚群中GPI-AP的表达量，建立了它们的正常表达模式，并为确定GPI-AP和PB细胞亚群的最佳组合以应用于PNH的诊断和监测提供了参考。我们的研究结果表明，不同的GPI-AP在不同的正常PB细胞亚群的表达模式的变量。CD 55和CD 59的联合使用代表了最有用的双标记组合;然而，其效用对于几个白细胞亚群和血小板仍然是次优的。对于某些细胞亚群，例如中性粒细胞，可以从相对广泛的组中选择另外的有用标记物（CD 16/CD 24/CD 55/CD 59/CD 66 b/CD 157），而对于其他细胞亚群，有用抗原的数量受到限制，（单核细胞：CD 14/CD 55/CD 157; B细胞：CD 24/CD 48/CD 52/CD 55; CD 4（+）T细胞：CD 48/CD 52/CD 55;嗜酸性粒细胞：CD 55/CD 59; CD 8（+）T细胞：CD 48/CD 55）或限于单一标志物（CD 56（低）NK细胞上的CD 48、BDCA 3（-）树突细胞和CD 56（高）NK细胞上的CD 55以及红细胞上的CD 59）。(c)2006年国际分析细胞学学会。
Background: Evaluation of the expression of glycosylphosphatidylinositol-anchored membrane proteins (GPI-AP) is currently used for the diagnosis of paroxysmal nocturnal hemoglobinuria (PNH). In this study, we analyzed the amount of expression of a wide variety of GPI-AP in different subsets of hematopoietic cells present in normal peripheral blood (PB), to establish their normal patterns of expression and provide a frame of reference for the definition of the best combination of GPI-AP and PB cell subsets to be applied in the diagnosis and monitoring of PNH.Results: Our results show variable patterns of expression of different GPI-AP in distinct subsets of normal PB cells. Combined use of CD55 and CD59 represented the most useful dual-marker combination; however, its utility remained suboptimal for several subsets of leukocytes and for platelets.Conclusions: For some cell subsets such as the neutraphils additional useful markers could be selected from a relatively broad panel (CD16/CD24/CD55/CD59/CD66b/CD157), whereas for other cell subsets the number of useful antigens was either restricted (monocytes: CD14/CD55/CD157; B cells: CD24/CD48/CD52/CD55; CD4(+) T cells: CD48/CD52/CD55; eosinophils: CD55/CD59; CD8(+) T cells: CD48/CD55) or limited to a single marker (CD48 on CD56(low) NK cells, CD55 on BDCA3(-) dendritic cells and CD56(high) NK cells, and CD59 for red cells), from all antigens analyzed. (c) 2006 International Society for Analytical Cytology.