Extensive and complex HIV-1 recombination between B′, C and CRF01_AE among IDUs in south-east Asia
Extensive and complex HIV-1 recombination between B′, C and CRF01_AE among IDUs in south-east Asia
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DOI：
10.1097/qad.0b013e3283522c97
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发表时间：
2012-06-01
期刊：
影响因子：
3.8
通讯作者：
Zheng, Yong-Tang
中科院分区：
文献类型：
--
作者：
Pang, Wei;Zhang, Chiyu;Zheng, Yong-Tang
目的：了解缅北地区注射吸毒者HIV-1亚型特征。设计：对缅北拉扎市和麦扎央市注射吸毒者进行分子流行病学调查。方法：收集2009年6月至8月同意注射吸毒者的83份HIV-1阳性血清，对HIV-1 p17、pol、vif-env、C2V3片段进行扩增和测序。进行了系统发育和引导扫描分析。结果：在该 HIV 感染的 IDU 队列中发现了非常高比例 (86.1%) 的 HIV-1 亚型间重组体和非常低比例的 B' 亚型 (3.8%)、C (7.6%) 和 CRF01_AE (1.3%)。这些重组体涵盖了CRF01_AE、B和C之间形成的所有四种重组形式。B/C和CRF01_AE/B/C重组体是最主要的两种重组体，分别占所有病例的54.4%和42.6%，表明缅甸北部CRF01_AE、B'和C之间正在产生广泛而复杂的HIV-1重组。有趣的是，大多数重组体具有彼此不同的嵌合模式，形成 64 种独特的重组形式 (URF)，与之前在亚洲发现的任何循环重组形式 (CRF) 和 URF 截然不同。结论：亚型间重组体的比例极高，特别是 CRF01_AE/B'/C 重组体 (42.6%)，强烈表明缅甸北部是 CRF01_AE、HIV-1 重组的大熔炉。 B' 和 C. (C) 2012 Wolters Kluwer Health 垂直条 Lippincott Williams & Wilkins
Objective: To investigate the subtype characterization of HIV-1 among IDUs in northern Myanmar.Design: A molecular epidemiological investigation was conducted among IDUs in Laza and Maizayang cities of northern Myanmar.Methods: A total of 83 HIV-1-positive serums were collected from consenting IDUs during June to August 2009. HIV-1 p17, pol, vif-env, C2V3 fragments were amplified and sequenced. Phylogenetic and bootscanning analyses were performed.Results: A very high proportion (86.1%) of HIV-1 intersubtype recombinants and very low proportion of subtypes B' (3.8%), C (7.6%) and CRF01_AE (1.3%) were found in this HIV-infected IDUs cohort. These recombinants cover all four kinds of recombination forms formed among CRF01_AE, B and C. The B/C and CRF01_AE/B/C recombinants are the two most dominant recombinants, accounting for 54.4 and 42.6% of all cases, respectively, and indicating the ongoing generation of extensive and complex HIV-1 recombination among CRF01_AE, B' and C in northern Myanmar. Intriguingly, most recombinants have different chimeric patterns from each other, forming 64 unique recombination forms (URFs) that are quite distinct from any previously identified circulating recombinant forms (CRFs) and URFs in Asia.Conclusion: The extremely high proportion of intersubtype recombinants, especially CRF01_AE/B'/C recombinants (42.6%), strongly suggests that northern Myanmar is a big forge for HIV-1 recombination among CRF01_AE, B' and C. (C) 2012 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins