Human rhinoviruses in INDIS-study material-evidence for recovery of viable rhinovirus from fecal specimens
Human rhinoviruses in INDIS-study material-evidence for recovery of viable rhinovirus from fecal specimens
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DOI：
10.1002/jmv.23593
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发表时间：
2013-08-01
影响因子：
12.7
通讯作者：
Roivainen, Merja
中科院分区：
文献类型：
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作者：
Savolainen-Kopra, Carita;Simonen-Tikka, Marja-Leena;Roivainen, Merja
近年来，随着分子检测方法的实施，人类鼻病毒（HRV）作为主要呼吸道病原体的重要性日益凸显。鼻病毒在不同严重程度的呼吸道感染住院病例中检出率很高，而且在普通感冒病例中也很常见。本研究的目的是评估前瞻性研究材料中 HRV 的发生情况。前瞻性 INDIS 材料包括在任何临床感染期间收集的 11 岁以下儿童的鼻咽 (N=429) 和粪便 (N=425) 标本。应用经过验证的实时 RT-PCR 检测来检测 HRV。不仅在鼻咽标本中检测到大量 HRV，在粪便标本中也检测到大量 HRV，分别为 236 例（55.0%）和 149 例（35.1%），粪便中的发现实际上超出了预期。共选取13份HRV阳性粪便标本进行VP4/VP2编码区基因分型。在七个样本中检测到 HRV-A 菌株：HRV-A9、-A10、-A24、-A49、-A56 和 -A82。 HRV-B 株检测了 3 次：HRV-B42 和 -B79，HRV-C 检测了两次：HRV-C12 和 HRV-Cpat4。 HRV-B42 在细胞培养物中也显示出细胞病变效应，经实时 RT-PCR 和 VP4/VP2 测序证实，表明粪便样本中存在活 HRV。 (c) 2013 年 Wiley 期刊公司。
The significance of human rhinoviruses (HRV) as prevailing respiratory pathogens has sharpened during the recent years followed by implementation of molecular methods in detection. Rhinoviruses are detected exceedingly in hospitalized cases of respiratory infection with varying severity, in addition to being frequent in cases of common cold. The aim of this study was to evaluate occurrence of HRV in a prospective study material. The prospective INDIS material comprises nasopharyngeal (N=429) and fecal (N=425) specimens from children under 11 years of age collected during any clinical infection. Validated real-time RT-PCR assays were applied for the detection of HRV. HRV were detected numerously not only in the nasopharyngeal specimens, but a myriad also in fecal specimens, 236 (55.0%) and 149 (35.1%), respectively, fecal findings actually beyond anticipation. A total of 13 of HRV-positive fecal specimens were selected for genetic typing in the VP4/VP2 coding region. HRV-A strains were detected in seven specimens: HRV-A9, -A10, -A24, -A49, -A56 and -A82. HRV-Bstrains were detected three times: HRV-B42 and -B79, and HRV-C twice: HRV-C12 and HRV-Cpat4. HRV-B42 also showed cytopathic effect in cell culture, confirmed by real-time RT-PCR and VP4/VP2 sequencing, suggesting presence of viable HRV in fecal specimens. (c) 2013 Wiley Periodicals, Inc.