Self-assembled CNTs/CdS/dehydrogenase hybrid-based amperometric biosensor triggered by photovoltaic effect.
Self-assembled CNTs/CdS/dehydrogenase hybrid-based amperometric biosensor triggered by photovoltaic effect.
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DOI：
10.1016/j.bios.2008.03.043
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发表时间：
2008-10
影响因子：
12.6
通讯作者：
L. Tang;Yihua Zhu;Xiaoling Yang;Jinjie Sun;Chunzhong Li
中科院分区：
文献类型：
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作者：
L. Tang;Yihua Zhu;Xiaoling Yang;Jinjie Sun;Chunzhong Li
一种新型多组分混合材料，即在多壁碳纳米管（CNT）上自组装量子点（CdS）和谷氨酸脱氢酶（GDH），设计用于电流生物传感系统。电位和透射电子显微镜 (TEM) 分析证实了 CdS/GDH 在羧基功能化 CNT 上的均匀生长。与单一的 CdS 相比，所得的混合材料在光照下表现出更有效的光电流产生。入射光激发 CdS 并产生电荷载流子，然后 CNT 促进电荷转移。对于基于脱氢酶的生物传感器，通常需要β-烟酰胺腺嘌呤二核苷酸（NAD+）或β-烟酰胺腺嘌呤二核苷酸磷酸（NADP+）的辅因子。此外，我们发现CNTs/CdS/GDH的光伏效应可以在NAD+或NADP+辅因子不存在的情况下触发脱氢酶反应。电化学实验结果还表明，非辅因子依赖性脱氢酶生物传感系统具有良好的灵敏度（11.9nA/μM）、较低的检测限（高达50nM）、可接受的重现性和稳定性等一系列吸引人的特性。这些研究有助于理解半导体纳米杂化物（碳纳米管/量子点等）与生物分子（酶等）的结合，在生物传感器、生物燃料电池、生物医学和其他生物电子领域具有应用潜力。
A novel multi-components hybrid material, self-assembled quantum dots (CdS) and glutamate dehydrogenase (GDH) onto multiwall carbon nanotubes (CNTs), was designed for amperometric biosensing system. The ζ-potential and transmission electron microscopy (TEM) analyses confirmed the uniform growth of the CdS/GDH onto carboxyl-functionalized CNTs. Compared with the single CdS, the resulting hybrid material showed more efficient generation of photocurrent upon illumination. The incident light excites CdS and generates charge carriers, and then CNTs facilitates the charge transfer. For dehydrogenase-based biosensor, normally, the cofactor of β-nicotinamide adenine dinucleotide (NAD+) or β-nicotinamide adenine dinucleotide phosphate (NADP+) is necessary. Furthermore, we found the photovoltaic effect of CNTs/CdS/GDH can trigger the dehydrogenase enzymatic reaction in the absence of the NAD+or NADP+cofactors. The electrochemical experiment results also demonstrate that the cofactor-independent dehydrogenase biosensing system had series attractive characteristics, such as a good sensitivity (11.9nA/μM), lower detection limit (up to 50nM), an acceptable reproducibility and stability. These studies aid in understanding the combination of the semiconductor nanohybrids (CNTs/QDs, etc.) and biomolecules (enzymes, etc.), which has potential for the applications in biosensor, biofuel cell, biomedical and other bioelectronics field.