Sepsis induces muscle atrophy by inhibiting proliferation and promoting apoptosis via PLK1-AKT signalling.
Sepsis induces muscle atrophy by inhibiting proliferation and promoting apoptosis via PLK1-AKT signalling.
复制标题
脓毒症通过PLK 1-AKT信号传导抑制增殖和促进凋亡诱导肌肉萎缩。
DOI：
10.1111/jcmm.16921
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发表时间：
2021-10
影响因子：
5.3
通讯作者：
Yu JB
中科院分区：
文献类型：
--
作者：
Cao YY;Wang Z;Yu T;Zhang Y;Wang ZH;Lu ZM;Lu WH;Yu JB
Sepsis and sepsis‐induced skeletal muscle atrophy are common in patients in intensive care units with high mortality, while the mechanisms are controversial and complicated. In the present study, the atrophy of skeletal muscle was evaluated in sepsis mouse model as well as the apoptosis of muscle fibres. Sepsis induced atrophy of skeletal muscle and apoptosis of myofibres in vivo and in vitro. In cell‐based in vitro experiments, lipopolysaccharide (LPS) stimulation also inhibited the proliferation of myoblasts. At the molecular level, the expression of polo‐like kinase 1 (PLK1) and phosphorylated protein kinase B (p‐AKT) was decreased. Overexpression of PLK1 partly rescued LPS‐induced apoptosis, proliferation suppression and atrophy in C2C12 cells. Furthermore, inhibiting the AKT pathway deteriorated LPS‐induced atrophy in PLK1‐overexpressing C2C12 myotubes. PLK1 was found to participate in regulating apoptosis and E3 ubiquitin ligase activity in C2C12 cells. Taken together, these results indicate that sepsis induces skeletal muscle atrophy by promoting apoptosis of muscle fibres and inhibiting proliferation of myoblasts via regulation of the PLK1‐AKT pathway. These findings enhance understanding of the mechanism of sepsis‐induced skeletal muscle atrophy.
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影响因子：
3.8
作者：
Kizilboga, Tugba;Baskale, Emine Arzu;Dinler-Doganay, Gizem
通讯作者：
Dinler-Doganay, Gizem
影响因子：
5.7
作者：
Gutteridge RE;Ndiaye MA;Liu X;Ahmad N
通讯作者：
Ahmad N
影响因子：
16.6
作者：
Milan, Giulia;Romanello, Vanina;Pescatore, Francesca;Armani, Andrea;Paik, Ji-Hye;Frasson, Laura;Seydel, Anke;Zhao, Jinghui;Abraham, Reimar;Goldberg, Alfred L.;Blaauw, Bert;DePinho, Ronald A.;Sandri, Marco
通讯作者：
Sandri, Marco
影响因子：
3.4
作者：
Lee, Gangpyo;Lim, Jae-Young;Frontera, Walter R.
通讯作者：
Frontera, Walter R.
影响因子：
5.2
作者：
Fink MP
通讯作者：
Fink MP