CHARACTERIZATION OF A CORONAVIRUS .2. GLYCOPROTEINS OF VIRAL ENVELOPE - TRYPTIC PEPTIDE ANALYSIS
CHARACTERIZATION OF A CORONAVIRUS .2. GLYCOPROTEINS OF VIRAL ENVELOPE - TRYPTIC PEPTIDE ANALYSIS
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DOI：
10.1016/0042-6822(77)90489-5
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发表时间：
1977-01-01
期刊：
影响因子：
3.7
通讯作者：
HOLMES, KV
中科院分区：
文献类型：
--
作者：
STURMAN, LS;HOLMES, KV
在冠状病毒粒子中鉴定出两种膜相关糖蛋白。它们很容易根据大小、放射性标记特征和相对于脂质双层的位置进行区分。较大的糖蛋白被放射性标记的岩藻糖和葡糖胺高度标记。该物种有两种形式，GP 180和GP 90，表观分子量分别为180，000和90，000。通过用胰蛋白酶处理病毒体，GP 180可以在体外转化为GP 90。GP 90和GP 180的胰蛋白酶解产物的分析给出相同的肽模式。基于链霉蛋白酶和菠萝蛋白酶的敏感性，GP 180/90是唯一完全位于病毒包膜外部的蛋白质。它似乎包括病毒粒子特征性的长的、花瓣状的peplomers。较小的糖蛋白GP 23具有23，000的表观分子量，并且被放射性标记的葡糖胺标记，但不被岩藻糖标记。GP 23的葡糖胺标记水平为是GP 180/90的1/10。GP 23似乎具有2个不同的结构域：在病毒包膜外发现的较小的含碳水化合物的区域，以及由甲硫氨酸高度标记的较大部分，其与病毒膜整体相关。对3种主要的冠状病毒结构蛋白提出了新的命名。两种包膜糖蛋白GP 23和GP 180/90分别命名为E1和E2;内核蛋白VP 50命名为N。
Two species of membrane-associated glycoproteins were identified in the coronavirus virion. They are readily distinguished on the basis of size, radiolabeling characteristics and location in relation to the lipid bilayer. The larger glycoprotein is highly labeled by both radiolabeled fucose and glucosamine. This species is found in 2 forms, GP180 and GP90, with apparent MW of 180,000 and 90,000, respectively. GP180 can be converted to GP90 in vitro by treatment of virions with trypsin. Analysis of tryptic digests of GP90 and GP180 give identical peptide patterns. Based on pronase and bromelain sensitivities, GP180/90 is the only protein which is located entirely external to the viral envelope. It appears to comprise the characteristic long, petal-shaped, peplomers of the virion. The smaller glycoprotein, GP23, has an apparent MW of 23,000 and is labeled by radiolabeled glucosamine but not by fucose. The level of glucosamine-labeling of GP23 is .apprx. 1/10 that of GP180/90. GP23 appears to possess 2 distinct domains: a smaller, carbohydrate containing region which is found outside the viral envelope, and a larger portion, highly labeled by methionine, which is integrally associated with the viral membrane. A new nomenclature is proposed for the 3 major coronavirus structural proteins. The 2 envelope glycoproteins GP23 and GP180/90 are designated E1 and E2, respectively; the inner core protein VP50 is designated N.