THE CDC9 LIGASE JOINS COMPLETED REPLICONS IN BAKERS-YEAST
THE CDC9 LIGASE JOINS COMPLETED REPLICONS IN BAKERS-YEAST
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DOI：
10.1007/bf00330657
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发表时间：
1983-01-01
期刊：
影响因子：
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通讯作者：
JOHNSTON, LH
中科院分区：
文献类型：
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作者：
JOHNSTON, LH
药物羟基脲以与野生型相同的方式影响条件DNA连接酶突变体cdc9。特定浓度抑制完整复制子的连接，导致这些分子的大量积累。在去除羟基脲并在允许温度下进一步孵育cdc9细胞后，复制子连接在一起，而在限制温度下则没有形成鲜明对比。然而，能够在限制性温度下生长的cdc9回复突变体也能够在这些条件下连接复制子，因此cdc9连接酶必须负责完成复制子的组装。
The drug hydroxyurea affected the conditional DNA ligase mutant cdc9 in the same way as a wild type. Specific concentrations inhibit the joining of completed replicons leading to a substantial accumulation of these molecules. Upon removal of hydroxyurea and further incubation of cdc9 cells at the permissive temperature the replicons joined together, while in sharp contrast at the restrictive temperature no such joining occurred. However, a revertant of cdc9 able to grow at the restrictive temperature was also able to join replicons under these conditions, so the cdc9 ligase must be responsible for the assembly of completed replicons.