Theory of Weakly Polydisperse Cytoskeleton Filaments.
Theory of Weakly Polydisperse Cytoskeleton Filaments.
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随着肌动蛋白/微管蛋白的数量密度、结合剂的数量密度和类型以及电解质浓度的变化，细胞骨架细丝具有动态改变构象的非凡能力。这一特性对于真核细胞在不同的细胞区室中实现特定的生物学功能至关重要。传统的生物聚合物溶液方法对于细胞骨架细丝来说是失败的，因为它们需要几种近似的方法来处理它们的聚电解质和机械性能。在本文中，我们介绍了一种新的密度泛函理论的多分散，半柔性细胞骨架细丝。该方法考虑了平衡聚合动力学、丝的长度和取向分布以及丝与电解质之间的静电相互作用。这对于细胞骨架聚合在不同的细胞区室中产生不同长度的细丝是必不可少的，有时长到足以变得半柔性。我们表征了肌动蛋白丝在电解质水溶液中的热力学性质。我们计算了每个细丝构象的自由能、压强、化学势和二次维里系数。我们还计算了肌动蛋白丝溶液的相图，并与体外实验结果进行了比较。
Cytoskeleton filaments have the extraordinary ability to change conformations dynamically in response to alterations of the number density of actins/tubulin, the number density and type of binding agents, and the electrolyte concentration. This property is crucial for eukaryotic cells to achieve specific biological functions in different cellular compartments. Conventional approaches to biopolymers’ solution break down for cytoskeleton filaments because they entail several approximations to treat their polyelectrolyte and mechanical properties. In this article, we introduce a novel density functional theory for polydisperse, semiflexible cytoskeleton filaments. The approach accounts for the equilibrium polymerization kinetics, length and orientation filament distributions, as well as the electrostatic interaction between filaments and the electrolyte. This is essential for cytoskeleton polymerization in different cell compartments generating filaments of different lengths, sometimes long enough to become semiflexible. We characterized the thermodynamics properties of actin filaments in electrolyte aqueous solutions. We calculated the free energy, pressure, chemical potential, and second virial coefficient for each filament conformation. We also calculated the phase diagram of actin filaments’ solution and compared with the corresponding results in in vitro experiments.
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影响因子：
5.5
作者：
HENTSCHKE, R
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