Impact of polysol, a newly developed preservation solution, on cold storage of steatotic rat livers
Impact of polysol, a newly developed preservation solution, on cold storage of steatotic rat livers
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DOI：
10.1002/lt.20957
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发表时间：
2007-01-01
影响因子：
4.6
通讯作者：
Minor, Thomas
中科院分区：
文献类型：
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作者：
Hata, Koichiro;Tolba, Rene Hany;Minor, Thomas
供体器官的长期短缺导致接受脂肪变性肝脏作为移植物，尽管原发性移植物功能障碍的风险更高。我们在此报告了新开发的保存液Polysol对脂肪变性大鼠肝脏冷藏的有益影响。Wistar大鼠禁食2天，随后再喂食3天无脂肪、富含碳水化合物的饮食，诱导饮食性肝脂肪变性。取出脂肪肝，冲洗，然后用HTK或Polysol在4 ℃下储存24小时。两组移植物的功能完整性通过在37 ℃下用含氧Krebs-Henseleit缓冲液隔离再灌注45分钟来评价。Polysol保存不仅导致实质（AST（IU/L）; HTK中6728 +/- 824 vs. Polysol中3107 +/- 718; P < 0.001）而且导致线粒体（GLDH（IU/L）; 3189 +/- 773 vs. 1282 +/- 365; P < 0.01）酶释放在整个再灌注期间显著减少。此外，PVP（16.9 +/- 2.7对比7.8 +/-1.5mmHg; P < 0.05），肝脏O2消耗（0.291 +/- 0.047 vs. 1.056 +/- 0.053 μ mol/g肝/min; P < 0.001），组织ATP含量（0.695 +/- 0.086 vs. 1.340 +/- 0.157 μ mol/g干肝; P < 0.005），胆汁生成（0.79 +/- 0.11 vs. 4.08 +/- 0.66 mu L/g肝脏/45分钟; P < 0.001），丙二醛进入灌注液Polysol能更好地保存肝脏组织的蛋白质（1.922 +/- 0.198 vs. 0.573 +/- 0.094 nmol/L; P < 0.0001）和湿/干重比（5.20 +/- 0.31 vs. 3.85 +/- 0.15; P < 0.005）。与这些益处一致，电子显微镜显示Polysol保存基本上抑制了脂肪变性肝脏中有害的线粒体改变。总之，使用Polysol冷藏导致脂肪变性肝脏的完整性和功能显著更好。因此，Polysol可能是一种新的替代品，特别是对于“边缘”器官。
Chronic shortage of donor organs has led to acceptance of steatotic livers as grafts, although there is a higher risk of primary graft dysfunction. We herein report the beneficial impact of Polysol, a newly developed preservation solution, on cold storage of steatotic rat livers. Dietary hepatic steatosis was induced in Wistar rats by 2-day fasting and subsequent 3-day re-feeding with a fat-free, carbohydrate-rich diet. Fatty livers were retrieved, flushed and then stored at 4 degrees C for 24 hours with either HTK or Polysol. Functional integrity of the grafts was evaluated by isolated reperfusion with oxygenated Krebs-Henseleit buffer at 37 degrees C for 45 minutes in both groups. Polysol preservation resulted in significant reductions of not only parenchymal (AST (IU/L); 6728 +/- 824 in HTK vs. 3107 +/- 718 in Polysol; P < 0.001) but also mitochondrial (GLDH (IU/L); 3189 +/- 773 vs. 1282 +/- 365; P < 0.01) enzyme release throughout reperfusion. Moreover, PVP (16.9 +/- 2.7 vs. 7.8 +/- 1.5 mmHg; P < 0.05), hepatic 02 consumption (0.291 +/- 0.047 vs. 1.056 +/- 0.053 mu mol/g liver/min; P < 0.001), tissue ATP content (0.695 +/- 0.086 vs. 1.340 +/- 0.157 mu mol/g dry-liver; P < 0.005), bile production (0.79 +/- 0.11 vs. 4.08 +/- 0.66 mu L/g liver/45-min; P < 0.001), malondialdehyde into the perfusate (1.922 +/- 0.198 vs. 0.573 +/- 0.094 nmol/L; P < 0.0001) and wet/dry-weight ratio of the liver tissues (5.20 +/- 0.31 vs. 3.85 +/- 0.15; P < 0.005) were all better preserved by Polysol. In line with these benefits, electron microscopy revealed that Polysol preservation substantially suppressed deleterious mitochondrial alterations in steatotic livers. In conclusion, cold storage using Polysol resulted in significantly better integrity and function of steatotic livers. Polysol, therefore, may be a new alternative especially for "marginal" organs.