Stimulation of rat mesangial cell proliferation by macrophage interleukin 1.
Stimulation of rat mesangial cell proliferation by macrophage interleukin 1.
复制标题
巨噬细胞白细胞介素1刺激大鼠系膜细胞增殖。
作者：
D. Lovett;J. Ryan;R. Sterzel
lps激活的大鼠腹膜巨噬细胞条件培养基可提高培养的大鼠肾小球系膜细胞的增殖率。通过顺序硫酸铵沉淀、S-200凝胶层析、deae -纤维素阴离子交换层析和苯基- sepharose层析，广泛共纯化巨噬细胞来源的活性与白细胞介素1 （IL - 1）活性。此外，巨噬细胞衍生因子热不稳定（80℃），并被苯乙二醛灭活，因此可以初步鉴定为IL 1。巨噬细胞上清液和纯化的IL - 1仅在血清存在的情况下才能提高系膜细胞的增殖率；使用血小板贫乏的血浆或耗尽血小板衍生生长因子的血清无效果。IL - 1的作用是增加循环细胞的百分比，而不改变单个细胞周期时间的长度。这些发现提供了活化的巨噬细胞与血小板因子联合促进系膜细胞增殖的潜在机制。这些过程可能导致免疫介导的肾小球肾炎中常见的系膜细胞增多。
Conditioned media from LPS-activated rat peritoneal macrophages enhanced the proliferation rates of cultured rat glomerular mesangial cells. This macrophage-derived activity extensively co-purified with interleukin 1 (IL 1) activity through sequential ammonium sulfate precipitation, S-200 gel chromatography, DEAE-cellulose anion exchange chromatography, and phenyl-Sepharose chromatography. In addition, the macrophage-derived factor was heat-labile (80 degrees C) and inactivated by phenylglyoxal, thus allowing tentative identification as IL 1. Macrophage supernatants and purified IL 1 enhanced the proliferative rates of mesangial cells only in the presence of serum; the use of platelet-poor plasma or serum depleted of platelet-derived growth factor was without effect. IL 1 acted to increase the percentage of cycling cells, without a change in the length of the individual cell cycle times. These findings provide a potential mechanism whereby activated macrophages, in combination with platelet factors, enhance mesangial cell proliferation. Such processes may contribute to the mesangial hypercellularity frequently found in immune-mediated glomerulonephritis.