Full-length sequence and mosaic structure of a human immunodeficiency virus type 1 isolate from Thailand
Full-length sequence and mosaic structure of a human immunodeficiency virus type 1 isolate from Thailand
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DOI：
10.1128/jvi.70.9.5935-5943.1996
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发表时间：
1996-09-01
影响因子：
5.4
通讯作者：
McCutchan, FE
中科院分区：
文献类型：
--
作者：
Carr, JK;Salminen, MO;McCutchan, FE
包膜基因E的1型人类免疫缺陷病毒分离株对全球大流行有很大贡献，这些毒株似乎是嵌合体，其Gag基因来自A分支，包膜来自E分支；首次报道了CM240泰国分离物CM240的基因组全序列，在CM240病毒中发现了两个亲本之间的多个断裂点，整个Gag-pol区和大部分(如果不是全部)辅助基因vif、vpr、tat、rev和vPU似乎都来自A分支。在gp41的信号肽之后不久，该基因切换到E并返回到A分支；因此，位于细胞质一侧的包膜部分似乎主要不是像以前认为的那样来自E分支，而是来自A分支，在长末端重复序列中发现了另一小段不属于任何已知分支并可能也由亲本E株贡献的片段，可能重要的是隐含的病毒结构类似于一种伪型病毒，其基质和核心来自一个分支，外膜来自另一个分支。在长末端重复序列中，CM240在核因子-kappaB结合位点的数量、TATA盒的序列以及反式激活反应区茎环的二级结构等方面与其他分支存在差异。CM240病毒粒子的马赛克结构暗示了表型差异，这可能是导致该变体出现的原因之一。
Human immunodeficiency virus type 1 isolates of envelope genotype E are contributing substantially to the global pandemic, These strains appear to be mosaics, with the gag gene from clade A and the envelope from clade E; the parental clade E strain has not been found, Here we report the first full genomic sequence of one such mosaic virus, isolate CM240 from Thailand, Multiple breakpoints between the two parental genotypes have been found in a CM240 virus, The entire gag-pol region and most, if not all, of the accessory genes vif, vpr, tat, rev, and vpu appear to derive from clade A. The genotype switches to E shortly after the signal peptide of the envelope and back to clade A near the middle of gp41; thus, the portion of the envelope that lies on the cytoplasmic side of the membrane appears to be principally derived not from clade E, as previously thought, but from clade A, Another small segment not belonging to any recognized clade and presumably also contributed by the parental E strain has been found in the long terminal repeat, It may be significant that the implied virion structure resembles a pseudotype virus with the matrix and core from one clade and the outer envelope from another. In the long terminal repeat, differences were observed between CM240 and other clades in the number of NF-kappa B binding sites, the sequence of the TATA box, and the putative secondary structure of the transactivation response region stem-loop. The mosaic structure of a CM240 virion is suggestive of phenotypic differences which might have contributed to the emergence of this variant.