Evaluation of three PCR assays for the identification of the sheep strain (Genotype 1) of Echinococcus granulosus in canid feces and parasite tissues
Evaluation of three PCR assays for the identification of the sheep strain (Genotype 1) of Echinococcus granulosus in canid feces and parasite tissues
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DOI：
10.4269/ajtmh.2008.78.777
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发表时间：
2008-05-01
影响因子：
3.3
通讯作者：
Craig, Philip S.
中科院分区：
文献类型：
--
作者：
Boufana, Belgees S.;Campos-Ponce, Maiza;Craig, Philip S.
使用组织和犬科动物粪便样本评价了3种PCR检测方法用于鉴定细粒棘球绦虫G1绵羊基因型的性能。Dinkel引物和Stefanic引物检测E.尸检犬粪便中颗粒体DNA的阳性率分别为73.7%和100%。Abbasi引物对E.细粒棘球绦虫感染的狗，但最具种特异性，交叉反应只与志贺氏棘球绦虫（组织90.9%，粪便75%）。Stefanic引物对E.颗粒状。Dinkel引物也表现出种间交叉反应活性（组织，63.6%;粪便，100%），但被发现是菌株特异性的E。颗粒体G1绵羊基因型。棘球蚴属物种和亚种变体的PCR检测的改进应依赖于使用保守性较低的基因和改进从粪便中提取的DNA的质量和数量的方案的开发。
The performance of 3 PCR assays for the identification of the G1 sheep genotype of Echinococcus granulosus was evaluated using tissue and canid fecal samples. The "Dinkel" and "Stefanic" primers were the most sensitive in detecting E. granulosus DNA in feces of necropsied dogs (73.7% and 100%, respectively). The "Abbasi" primers detected 52.6% of E. granulosus infected dogs but were the most species-specific, cross-reacting only with Echinococcus shiquicus (tissue 90.9%; feces 75%). The Stefanic primers were the least specific (tissue, 27.3%; feces, 25%) for E. granulosus. The Dinkel primers also showed inter-species cross-re activity (tissue, 63.6%; feces, 100%) but were found to be strain-specific for the E. granulosus G1 sheep genotype. Improvement of PCR tests for Echinococcus species and subspecific variants should rely on the use of less-conserved genes and development of protocols that improve the quality and quantity of DNA extracted from feces.