Phorbol ester activation of phospholipase D in human monocytes but not peripheral blood lymphocytes.
Phorbol ester activation of phospholipase D in human monocytes but not peripheral blood lymphocytes.
复制标题
佛波酯激活人单核细胞中的磷脂酶 D，但不激活外周血淋巴细胞。
DOI：
10.1016/0006-291x(89)92379-6
复制
发表时间：
1989
影响因子：
3.1
通讯作者：
Benedict,SH
中科院分区：
文献类型：
--
作者：
Kinsky,SC;Loader,JE;Benedict,SH
先前的报告表明，通过酶催化的转磷脂酰化反应（磷脂酰乙醇的形成）可以激活人外周血单个核细胞中的磷脂酶D。在本研究中，通过两种方法分离单核细胞来鉴定反应细胞。与先前的建议相反，结果表明，磷脂酯不会刺激正常淋巴细胞的磷脂酶D活性。因此，磷脂酰乙醇不是由T淋巴细胞（通过羊红细胞莲座形成分离）或T淋巴细胞和B淋巴细胞的混合物（通过离心洗脱分离）产生的。在相同的条件下，磷酯能够在含有主要单核细胞的组分中激活磷脂D。初步实验进一步表明，磷脂酯在人T细胞白血病系（MOLT-3、CEM、JURKAT、PEER）中不能诱导磷脂酶D活性，但在感染eb病毒的部分（但不是全部）B细胞系中可以。
Previous reports have shown that 12-O-tetradecanoylphorbol-13-acetate can activate phospholipase D in human peripheral blood mononuclear cells as measured by an enzyme-catalyzed transphosphatidylation reaction (phosphatidylethanol formation). In the present study, the mononuclear cells were fractionated by two procedures to identify the responsive cells. Contrary to earlier suggestions, the results indicate that phorbol ester does not stimulate phospholipase D activity in normal lymphocytes. Thus, phosphatidylethanol was not produced by T lymphocytes (isolated by sheep erythrocyte rosette formation) or by a mixture of T and B lymphocytes (isolated by centrifugal elutriation). Under the same conditions, phorbol ester was able to activate phospholipse D in fractions that contained predominately monocytes. Preliminary experiments have further shown that phorbol ester does not induce phospholipase D activity in human T cell leukemic lines (MOLT-3, CEM, JURKAT, PEER) but can do so in some, but not all, B cell lines that have been infected with Epstein-Barr virus.