Association of the maternal microbiome in Japanese pregnant women with the cumulative prevalence of dermatitis in early infancy: A pilot study from the Chiba study of Mother and Child Health birth cohort
Association of the maternal microbiome in Japanese pregnant women with the cumulative prevalence of dermatitis in early infancy: A pilot study from the Chiba study of Mother and Child Health birth cohort
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DOI：
10.1016/j.waojou.2019.100065
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发表时间：
2019-10
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影响因子：
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通讯作者：
Hiromi Tanabe;K. Sakurai;Tamotsu Kato;Y. Kawasaki;T. Nakano;Fumiya Yamaide;Naoko Taguchi-Atarashi;Masahiro Watanabe;Shingo Ochiai;H. Ohno;H. Fukuoka;N. Shimojo;C. Mori
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作者：
Hiromi Tanabe;K. Sakurai;Tamotsu Kato;Y. Kawasaki;T. Nakano;Fumiya Yamaide;Naoko Taguchi-Atarashi;Masahiro Watanabe;Shingo Ochiai;H. Ohno;H. Fukuoka;N. Shimojo;C. Mori
背景产前母体微生物组，包括肠道微生物群，已被认为会影响后代过敏的发生率。此外，婴儿早期的表皮屏障功能障碍已被归因于随后过敏的发展。我们假设产前微生物组可能会影响肠道微生物群，作为改变胎儿免疫发育的初始触发因素。母亲的微生物组成可能与皮炎的患病率在婴儿早期（DEI）的后代，导致随后的过敏symptoms.MethodsThis研究的一部分，千叶研究的母亲和儿童健康（C-MACH）出生队列，是在2013年开始，434名健康孕妇在< 13周的妊娠被招募。DEI在出生后4个月内进行评估，并使用问卷调查确定10个月大的婴儿的过敏症状。与母体微生物组相关的其他信息来自怀孕期间填写的问卷。粪便样本收集孕妇在12（n= 59）和32周（n= 58）的妊娠，这是用于肠道菌群分析使用条形码16 S rRNA基因sequencing. Results过敏的症状，特别是遗传性过敏，显示出较高的患病率在出生后10个月的DEI组。DEI的发生与家庭规模和养猫量呈负相关。DEI组妊娠12周时母体粪便中变形菌门的多样性和妊娠32周时母体粪便中放线菌门的相对丰度在妊娠的两个时间点均较低。此外，在产前粪便中的变形菌的多样性呈负相关，在12周的家庭规模，并与狗的所有权在两个妊娠timepoints.ConclusionsThe组成的母体微生物可能会影响过敏的风险在后代，甚至在出生前。此外，变形菌的多样性和放线菌在母体粪便中的相对丰度与DEI呈负相关，这可能与婴儿期过敏发展的风险有关。这种早期触发可能是婴儿期和儿童期过敏发展的良好预测因素。
BackgroundThe prenatal maternal microbiome, including the gut microbiota, has been suggested to influence the incidence of allergies in offspring. Moreover, epidermal barrier dysfunction in early infancy has been attributed to the development of subsequent allergies. We hypothesized that the prenatal microbiome may affect the gut microbiota, acting as an initial trigger to alter immune development in the foetus. The maternal microbial composition may be linked to the prevalence of dermatitis in early infancy (DEI) of the offspring, leading to subsequent allergic symptoms.MethodsThis study was conducted as part of the Chiba Study of Mother and Child Health (C-MACH) birth cohort that was initiated in 2013; 434 healthy pregnant women at < 13 weeks of gestation were recruited. DEI was assessed for up to 4 months after birth, and allergic symptoms were determined in 10-month-old infants using questionnaires. Other information related to the maternal microbiome was obtained from questionnaires filled out during pregnancy. Stool samples were collected from pregnant women at 12 (n= 59) and 32 weeks (n= 58) of gestation, which were used for gut microbiota analysis using barcoded 16S rRNA gene sequencing.ResultsSymptoms of allergy, especially of inherited allergies, show a higher prevalence at 10 months after birth in the DEI group. DEI occurrence was negatively correlated with family size and cat ownership. The diversity of Proteobacteria at 12 weeks of gestation and the relative abundance of Actinobacteria at 32 weeks of gestation in maternal feces were lower at both time points of gestation in the DEI group. In addition, the diversity of Proteobacteria in prenatal feces was negatively correlated with family size at 12 weeks, and with dog ownership at both gestational time points.ConclusionsThe composition of the maternal microbiome may influence the risk of allergies in offspring, even before birth. Furthermore, the diversity of Proteobacteria and the relative abundance of Actinobacteria in maternal feces were negatively associated with DEI, which may be associated with the risk of allergy development in infancy. This early trigger may be a good predictor of allergy development during infancy and childhood.